ENHANCEMENT OF HEMOLYTIC ACTIVITY OF 2 COMPONENT OF HUMAN COMPLEMENT BY OXIDATION
ENHANCEMENT OF HEMOLYTIC ACTIVITY OF 2 COMPONENT OF HUMAN COMPLEMENT BY OXIDATION
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DOI：
10.1084/jem.126.6.1013
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发表时间：
1967-01-01
影响因子：
15.3
通讯作者：
MULLEREB.HJ
中科院分区：
文献类型：
--
作者：
POLLEY, MJ;MULLEREB.HJ
本文描述了一种对人C[image]2进行化学修饰的方法，其结果是其溶血活性显著增强，并且用修饰后的C[image]2制备的中间复合物EAC[image][红细胞抗体补体]1a，4,2a的稳定性显著提高。这两种效应都可以用C[image] 3[补体的第三组分]转化酶C[image]4,2a的活性和稳定性的增加来充分解释，C[image]2经过修饰后生成。用临界浓度的碘处理C[image]2所产生的修饰可能包括分子内1个或多个巯基的氧化。
A method was described for the chemical modification of human C[image]2 which results in a pronounced enhancement of its hemolytic activity and a marked increase in the stability of the intermediate complex EAC[image] [erythrocyte antibody complement] 1a,4,2a prepared with the modified C[image]2. Both effects are fully explained by the observed increase in activity and stability of the C[image] 3 [3rd component of complement] converting enzyme, C[image]4,2a, following its generation with modified C[image]2. The modification resulting from treatment of C[image]2 with a critical concentration of iodine probably consists of oxidation of 1 or more sulfhydryl group within the molecule.