Assaying mechanosensation.

Assaying mechanosensation.
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DOI:
10.1895/wormbook.1.172.1
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发表时间:
2014-07-31
期刊:
WormBook : the online review of C. elegans biology
影响因子:
--
通讯作者:
Goodman MB
Goodman MB
中科院分区:
其他
文献类型:
--
作者:
Chalfie M;Hart AC;Rankin CH;Goodman MB

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C.秀丽隐杆线虫使用神经元电路来检测并响应各种机械刺激,该神经元电路已由C. elegans研究人员。在本章中,我们回顾和评论目前用于评估机械感觉反应的技术。这一方法审查的目的是作为一个介绍那些新的领域和方便的专家简编。简要讨论了常用的mechanosensory测定,沿着讨论的神经回路,考虑的关键协议的细节,并参考主要文献。
C. elegans detect and respond to diverse mechanical stimuli using neuronal circuitry that has been defined by decades of work by C. elegans researchers. In this WormMethods chapter, we review and comment on the techniques currently used to assess mechanosensory response. This methods review is intended both as an introduction for those new to the field and a convenient compendium for the expert. A brief discussion of commonly used mechanosensory assays is provided, along with a discussion of the neural circuits involved, consideration of critical protocol details, and references to the primary literature.