The RNA-binding protein Tsunagi interacts with Mago Nashi to establish polarity and localize oskar mRNA during Drosophila oogenesis.
The RNA-binding protein Tsunagi interacts with Mago Nashi to establish polarity and localize oskar mRNA during Drosophila oogenesis.
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DOI：
10.1101/gad.927001
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发表时间：
2001-11
影响因子：
10.5
通讯作者：
Stephanie E. Mohr;S. Dillon;Robert E. Boswell
中科院分区：
文献类型：
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作者：
Stephanie E. Mohr;S. Dillon;Robert E. Boswell
在黑腹果蝇中，轴和原始生殖细胞的形成是由生殖系衍生的卵母细胞和周围体细胞卵泡细胞之间的相互作用调节的。这种相互的信号传导导致了对这些产蛋过程至关重要的mrna和蛋白质的不对称定位。Mago Nashi蛋白解释后卵泡细胞到卵母细胞的信号，以建立主轴并决定原始生殖细胞的命运。利用酵母双杂交系统，我们已经确定了一种rna结合蛋白Tsunagi，它与Mago Nashi蛋白相互作用。蛋白质共免疫沉淀和共定位，表明它们在体内形成复合物。免疫定位显示，海啸蛋白在第1-5和第8-9期定位于卵母细胞后细胞质中，并且这种定位依赖于野生型mago nashi功能。当从生殖系中去除海啸功能时，卵室发育，卵母细胞核无法迁移，oskar mRNA不定位于后极，并且观察到背腹模式异常。这些结果表明，Mago Nashi-Tsunagi蛋白复合体是解释后卵泡细胞-卵母细胞信号、确定主要体轴和定位确定原始生殖细胞所需成分所必需的。
In Drosophila melanogaster, formation of the axes and the primordial germ cells is regulated by interactions between the germ line-derived oocyte and the surrounding somatic follicle cells. This reciprocal signaling results in the asymmetric localization of mRNAs and proteins critical for these oogenic processes. Mago Nashi protein interprets the posterior follicle cell-to-oocyte signal to establish the major axes and to determine the fate of the primordial germ cells. Using the yeast two-hybrid system we have identified an RNA-binding protein, Tsunagi, that interacts with Mago Nashi protein. The proteins coimmunoprecipitate and colocalize, indicating that they form a complex in vivo. Immunolocalization reveals that Tsunagi protein is localized within the posterior oocyte cytoplasm during stages 1-5 and 8-9, and that this localization is dependent on wild-type mago nashi function. When tsunagi function is removed from the germ line, egg chambers develop in which the oocyte nucleus fails to migrate, oskar mRNA is not localized within the posterior pole, and dorsal-ventral pattern abnormalities are observed. These results show that a Mago Nashi-Tsunagi protein complex is required for interpreting the posterior follicle cell-to-oocyte signal to define the major body axes and to localize components necessary for determination of the primordial germ cells.