MEASUREMENTS OF FAST LIGHT-INDUCED LIGHT-SCATTERING AND LIGHT-ABSORPTION CHANGES IN OUTER SEGMENTS OF VERTEBRATE LIGHT SENSITIVE ROD CELLS
MEASUREMENTS OF FAST LIGHT-INDUCED LIGHT-SCATTERING AND LIGHT-ABSORPTION CHANGES IN OUTER SEGMENTS OF VERTEBRATE LIGHT SENSITIVE ROD CELLS
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DOI：
10.1007/bf00535653
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
KREUTZ, W
中科院分区：
文献类型：
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作者：
HOFMANN, KP;UHL, R;KREUTZ, W
在380和800 nm处测量了闪光灯引起的青蛙和牛悬浮液中脊椎动物杆外节（ROS）光吸收和光散射的变化。所用的光度计允许在明确定义的角度下观察光强度的变化。我们研究了在一系列闪光中信号幅度的连续减小。本文讨论了以下结果：1.在380 nm处的信号是由变视紫红质Ⅱ的形成引起的吸收变化和一个双相附加信号的叠加。后者只存在于漂白的初始范围（15 - 25%视紫红质）。2.在800 nm处观察到三个散射信号，其特征在于它们的连续幅度减小和时间过程：N：具有与变视紫红质II吸收变化相当的时间过程和连续幅度减小的小信号，可能由盘膜内的结构变化引起。Ni：慢信号，随着第一次闪光消失，这可以理解为外膜效应。具有连续下降速率的双相信号，比变视紫红质II信号高10至20倍。这两个动力学上不同的部件通过观察角度的变化而分离。两个不同延伸的区域似乎随着不同的时间进程而发生结构性变化。“P”可以反映光诱导发射器释放对盘形状和/或质量的影响。
Flash-induced changes of light-absorption and of light-scattering of vertebrate rod outer segments (ROS) from frog and cattle in suspension were measured at 380 and 800 nm. The photometer used allows the observation of light intensity changes under well defined angles.We studied the successive decrease of the signal amplitude in series of flashes. One flash bleaches about 1% rhodopsin.The following results are discussed:1.The signal at 380 nm is a superposition of the absorption change caused by formation of metarhodopsin II and of a biphasic additional signal. The latter exists only for the initial range of bleaching (15 to 25% rhodopsin).2.At 800 nm three scattering signals are observed which are characterized by their successive amplitude decrease and time course:N:A small signal with time course and successive amplitude decrease comparable to the metarhodopsin II absorption change, probably arising from a structural change within the disc membrane.Ni:A slow signal, disappearing with the first flash, which may be understood as an outer membrane effect.P:A biphasic signal with a successive decrease rate, by a factor of 10 to 20 higher than that of the metarhodopsin II signal. The two kinetically different components are separated by variation of the observation angle. Two regions of different extension appear to change structurally with different time course. “P” may reflect an influence of the light-induced transmitter release on disc shape and/or mass.