Xenon-enhanced computed tomography assessment of brown adipose tissue distribution and perfusion in lean, obese, and diabetic primates.
Xenon-enhanced computed tomography assessment of brown adipose tissue distribution and perfusion in lean, obese, and diabetic primates.
复制标题
DOI：
10.1002/oby.23519
复制
发表时间：
2022-09
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究旨在验证氙增强计算机断层扫描（XECT）用于检测棕色脂肪组织（BAT），并使用XECT评估瘦、肥胖和糖尿病非人灵长类动物（NHP）之间BAT分布和灌注的差异。在BAT产热的肾上腺素能刺激期间，在麻醉的恒河猴和长尾猴中进行全身XECT成像。在XECT图像中，BAT被确定为脂肪组织，在氙吸入过程中，与皮下脂肪相比，BAT经历了显著的放射密度增强。为了测量BAT血流量，在氙吸入期间获得的六个计算机断层扫描上随时间测量BAT放射密度增强。死后免疫组化染色用于确认影像学结果。XECT能够正确识别在尸检时确认的所有BAT库，从而能够构建NHP中BAT的第一个全面解剖图。与肥胖动物和健康动物相比，在糖尿病动物中发现BAT灌注的显著减少，以及与肥胖和瘦动物相比，在糖尿病动物中没有腋窝BAT和锁骨上BAT的显著减少。在肥胖和糖尿病的NHP模型中使用XECT允许分析代谢状态对BAT质量和灌注的影响。
This study aimed to validate xenon‐enhanced computed tomography (XECT) for the detection of brown adipose tissue (BAT) and to use XECT to assess differences in BAT distribution and perfusion between lean, obese, and diabetic nonhuman primates (NHPs). Whole‐body XECT imaging was performed in anesthetized rhesus and vervet monkeys during adrenergic stimulation of BAT thermogenesis. In XECT images, BAT was identified as fat tissue that, during xenon inhalation, underwent significant radiodensity enhancement compared with subcutaneous fat. To measure BAT blood flow, BAT radiodensity enhancement was measured over time on the six computed tomography scans acquired during xenon inhalation. Postmortem immunohistochemical staining was used to confirm imaging findings. XECT was able to correctly identify all BAT depots that were confirmed at necropsy, enabling construction of the first comprehensive anatomical map of BAT in NHPs. A significant decrease in BAT perfusion was found in diabetic animals compared with obese animals and healthy animals, as well as absence of axillary BAT and significant reduction of supraclavicular BAT in diabetic animals compared with obese and lean animals. The use of XECT in NHP models of obesity and diabetes allows the analysis of the impact of metabolic status on BAT mass and perfusion.
影响因子：
3.7
作者：
Branca RT;Zhang L;Warren WS;Auerbach E;Khanna A;Degan S;Ugurbil K;Maronpot R
通讯作者：
Maronpot R