QTL MAPPING OF NATURALLY-OCCURRING VARIATION IN FLOWERING TIME OF ARABIDOPSIS-THALIANA
QTL MAPPING OF NATURALLY-OCCURRING VARIATION IN FLOWERING TIME OF ARABIDOPSIS-THALIANA
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DOI：
10.1007/bf00282217
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发表时间：
1994-12-01
期刊：
影响因子：
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通讯作者：
PATERSON, AH
中科院分区：
文献类型：
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作者：
KOWALSKI, SP;LAN, TH;PATERSON, AH
一架隔离的F-2？对晚花生态型汉诺威/Munden（HM）和早花生态型Wassilewskija（WS）杂交获得的拟南芥群体的开花时间和其它形态性状进行了分析。2个影响始花天数的非连锁数量性状基因座（DFF-a和DFF-b）定位于第5染色体。影响节数（NN）、开花时叶长（LLF）和35天叶长（LL 35）的QTL也定位在第5染色体上; LLF-a、LL 35-a、NN-a与DFF-a定位在第5染色体上相同的区域; LLF-b和LL 35-b与DFF-b定位在第5染色体上相同的区域。另一个影响开花时叶长的QTL（LLF-c）定位于第3染色体。DFF-a、LLF-a、LL 35-a和NN-a的接近性以及这些QTL之间基因作用的相似性（加性）表明它们可能是该位点上单个基因的多效性结果。类似地，LL 35-B和LLF-B图谱彼此接近，并且都显示隐性基因作用，再次表明多效性的可能性。DFF-B也定位在LL 35-B和LLF-B附近，主要显示加性基因作用（尽管不能排除隐性基因作用）。这表明DFF-B可能代表与LL 35-B和/或LLF-B不同的基因。DFF-a图谱靠近两个先前鉴定的突变体：co（也影响开花时间，并显示与加和性一致的基因作用）和flc。影响相关性状DFF、LLF、LL 35和NN的QTL的相似图谱位置和基因作用表明，这些基因组区域含有天然存在的等位基因变体，这些等位基因变体参与植物从营养生长到生殖生长的一般过渡。
A segregating F-2? population of Arabidopsis thaliana derived from a cross between the late-flowering ecotype Hannover/Munden (HM) and the early-flowering ecotype Wassilewskija (WS) was analyzed for flowering time and other morphological traits. Two unlinked quantitative trait loci (QTLs) affecting days to first flower (DFF-a and DFF-b) mapped to chromosome 5. QTLs which affect node number (NN), leaf length at flowering (LLF), and leaf length at 35 days (LL35) also mapped to chromosome 5; LLF-a, LL35-a, NN-a map to the same region of chromosome 5 as DFF-a; LLF-b and LL35-bmap to the same region of chromosome 5 as DFF-b. Another QTL affecting leaf length at flowering (LLF-c) maps to chromosome 3. The proximity of DFF-a, LLF-a, LL35-a and NN-a, as well as the similarity in gene action among these QTLs (additivity), suggest that they may be pleiotropic consequences of a single gene at this locus. Similarly, LL35-b and LLF-b map near each other and both display recessive gene action, again suggesting the possibility of pleiotropy. DFF-b, which also maps near LL35-b and LLF-b, displays largely additive gene action (although recessive gene action could not be ruled out). This suggests that DFF-b may represent a different gene from LL35-b and/or LLF-b. DFF-a maps near two previously identified mutants: co (which also affects flowering time and displays gene action consistent with additivity) and flc. Similar map locations and gene actions of QTLs affecting the correlated traits DFF, LLF, LL35 and NN suggest that these genomic regions harbor naturally occurring allelic variants involved in the general transition of the plant from vegetative to reproductive growth.