IN-SITU AND IN-VITRO SPECIFICITY BETWEEN RHIZOCTONIA SPP AND SPIRANTHES-SINENSIS (PERSOON) AMES VAR AMOENA (M BIEBERSTEIN) HARA (ORCHIDACEAE)
IN-SITU AND IN-VITRO SPECIFICITY BETWEEN RHIZOCTONIA SPP AND SPIRANTHES-SINENSIS (PERSOON) AMES VAR AMOENA (M BIEBERSTEIN) HARA (ORCHIDACEAE)
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DOI：
10.1111/j.1469-8137.1994.tb02974.x
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发表时间：
1994-08-01
期刊：
影响因子：
9.4
通讯作者：
KATSUYA, K
中科院分区：
文献类型：
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作者：
MASUHARA, G;KATSUYA, K
兰花 Spiranthes sinensis (Persoon) Ames 之间的关系。变种amoena (M. Bieberstein) Hara 和丝核菌属 spp.在发芽时和成年植物中进行了原位研究。将兰花种子放入棉纱布中，埋藏在兰花栖息地草皮草原的 210 个采样点（原位发芽）。八周后，210 个采样点中的 67 个证实了兰花的原球茎发育。从原球茎中分离真菌表明，原位萌发主要是由白丝核菌诱导的。同样，R. repens 是从成年植物根部分离出的优势菌根真菌。埋藏点半径30cm或50cm内的成株数量不影响种子萌发。丝核菌的分布。通过使用荞麦茎的诱饵方法对采样点中的除R. repens 以外的其他物种进行了检查。 32 个分离株，包括双核丝核菌吻合群 (AG)-A、AGB、AG-G 和 AG-I、R. solani Kuhn AG-4、Waitea circinata Warcup 和：与 WAG-O 和 WAG-Z 吻合的 Talbot，以及多核丝核菌 sp。被孤立了。从匍匐草诱导原球茎发育的点获得了三个 AG-G 分离株。 S. sinensis var. 种子在体外用这些分离株接种 amoena 以测试共生发芽。大多数丝核菌属。与原位萌发不相关，诱导种子在体外萌发。将在体外用这些分离株发育的幼苗转移至体外条件。随着幼苗持续生长 3 个月，新叶发育并伸长。种子埋藏方法能够澄清原位和体外兰花真菌特异性的差异。我们的结论是 S. sinensis var. 之间的特异性。阿莫埃纳和真菌的原位条件与体外不同。
The relationships between the orchid Spiranthes sinensis (Persoon) Ames. var. amoena (M. Bieberstein) Hara and Rhizoctonia spp. were investigated in situ at germination and in adult plants. Seeds of the orchid placed in cotton gauze were buried at 210 sampling points in turf grassland, the orchid habitat (in situ germination). Eight weeks later, protocorm development of the orchid was confirmed at 67 of the 210 sampling points. Isolation of fungi from protocorms showed that in situ germination was induced mainly by Rhizoctonia repens Bernard. Similarly, R. repens was the dominant mycorrhizal fungus isolated from roots of adult plants. The number of adult plants within a radius of either 30 or 50 cm of burial points did not influence seed germination.The distribution of Rhizoctonia spp. other than R. repens in the sample site was examined with a baiting method using buckwheat stems. Thirty-two isolates consisting of binucleate Rhizoctonia anastomosis group (AG)-A, AGB, AG-G, and AG-I, R. solani Kuhn AG-4, Waitea circinata Warcup and: Talbot, which anastomozed with WAG-O and WAG-Z, and a multinucleate Rhizoctonia sp. were isolated. Three AG-G isolates were obtained from the points at which protocorm development was induced by R. repens. Seeds of S. sinensis var. amoena were inoculated in vitro with these isolates to test for symbiotic germination. Most Rhizoctonia spp. not associated with the germination in situ induced seed germination in vitro. Seedlings which developed with these isolates in vitro were transferred to ex vitro conditions. New leaves developed and elongated as seedlings continued to grow for 3 months.The seed burial method enabled the clarification of the differences in orchid-fungal specificity in situ and in vitro. We concluded that the specificity between S. sinensis var. amoena and fungi in situ conditions was different to that in vitro.