Phosphorylation of Threonine343 Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency
Phosphorylation of Threonine343 Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency
复制标题
苏氨酸343的磷酸化对于OCT4与SOX2的相互作用在维持小鼠胚胎干细胞多能性方面至关重要
DOI：
10.1016/j.stemcr.2017.09.001
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发表时间：
2017-11-14
影响因子：
5.9
通讯作者：
Li, Lingsong
中科院分区：
文献类型：
--
作者：
Abulaiti, Xianmixinuer;Zhang, Han;Li, Lingsong
OCT4 是维持胚胎干细胞 (ESC) 多能性所必需的；然而，OCT4 的过量表达会诱导 ESC 向原始内胚层分化。这种分化开关的分子机制尚不完全清楚。在这里，我们发现苏氨酸（343）被丙氨酸（T343A）取代，而不是天冬氨酸（T343D），导致ESC中OCT4磷酸化信号的显着损失。这种 OCT4 磷酸化的缺失会损害其与 SOX2 的相互作用，但会促进与 SOX17 的相互作用。因此，我们认为基于苏氨酸（343）的 OCT4 磷酸化对于维持 ESC 多能性至关重要。这种基于 OCT4 磷酸化的机制可能有助于深入了解早期胚胎发育过程中谱系规范的调节。
OCT4 is required to maintain the pluripotency of embryonic stem cells (ESCs); yet, overdose-expression of OCT4 induces ESC differentiation toward primitive endoderm. The molecular mechanism underlying this differentiation switch is not fully understood. Here, we found that substitution of threonine(343) by alanine (T343A), but not aspartic acid (T343D), caused a significant loss of OCT4-phosphorylation signal in ESCs. Loss of such OCT4-phosphorylation compromises its interaction with SOX2 but promotes interaction with SOX17. We therefore propose that threonine(343)-based OCT4-phosphorylation is crucial for the maintenance of ESC pluripotency. This OCT4-phosphorylation-based mechanism may provide insight into the regulation of lineage specification during early embryonic development.