Circular genomes related to anelloviruses identified in human and animal samples by using a combined rolling-circle amplification/sequence-independent single primer amplification approach
Circular genomes related to anelloviruses identified in human and animal samples by using a combined rolling-circle amplification/sequence-independent single primer amplification approach
复制标题
DOI：
10.1099/vir.0.83071-0
复制
发表时间：
2007-10-01
影响因子：
3.8
通讯作者：
de Micco, Philippe
中科院分区：
文献类型：
--
作者：
Biagini, Philippe;Uch, Rathviro;de Micco, Philippe
结合滚环扩增（RCA）和序列独立的单引物扩增（SISPA）的方法应用于4个样品的人血浆和一个样品的唾液从猫。这种方法允许9个环节病毒的表征。它们中的大多数被鉴定为高度分歧的菌株，被归类为环节病毒属的种。对迄今为止在人类中描述的最小的环节病毒进行了表征（2 PoSMA，2002 nt;“小环节病毒”物种）。还在人血浆样品中鉴定了属于Torque Teno Mini病毒物种的两个高度不同的序列（LIL-y1，2887 nt; LIL-y2，2871 nt），其聚集成一个新的系统发育分支。最后，在猫的唾液中鉴定了两个基因组，它们之间的遗传差异为46%，其中一个产生了不同的系统发育分支（PRA 1，2019 nt）。这些结果突出了RCA-SISPA用于检测环状（或环状化）基因组的潜力。
A combined rolling-circle amplification (RCA) and sequence-independent single primer amplification (SISPA) approach was applied to four samples of human plasma and one sample of saliva from a cat. This approach permitted the characterization of nine anelloviruses. Most of them were identified as highly divergent strains that were classified into species of the genus Anellovirus. The smallest anellovirus described so far in humans was characterized (2PoSMA, 2002 nt; 'small anellovirus' species). Two highly divergent sequences belonging to the species Torque Teno Mini Virus (LIL-y1, 2887 nt; LIL-y2, 2871 nt), which clustered into a new phylogenetic branch, were also identified in human plasma samples. Finally, two genomes that are separated by a genetic divergence of 46% were characterized in the cat's saliva, one of these creating a distinct phylogenetic branch (PRA1, 2019 nt). These results highlight the potential of RCA-SISPA for detecting circular (or circularized) genomes.