Application of Deep Pyrosequencing to the Analysis of Soil Microbial Communities in Different Lotus Fields
Application of Deep Pyrosequencing to the Analysis of Soil Microbial Communities in Different Lotus Fields
复制标题
深度焦磷酸测序在不同荷田土壤微生物群落分析中的应用
DOI：
10.12783/issn.1544-8053/13/4/s31
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发表时间：
2016-01-01
影响因子：
--
通讯作者：
Sun, Xiaotang
中科院分区：
文献类型：
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作者：
Cui, Ruqiang;Wang, Hegui;Sun, Xiaotang
尖孢镰刀菌莲藕枯萎病菌是江西省重要的植物病原菌，可引起莲藕枯萎病，造成严重的经济损失。16S核糖体RNA基因和内转录间隔区序列分析结果表明，不同连作年限的健康和枯萎病病株莲田土壤中存在不同的细菌和真菌群落，表明莲的长期连作显著改变了土壤微生物群落。健康土壤样品中真菌多样性指数随单作年限的增加而增加，枯萎病土壤样品中真菌多样性指数随单作年限的增加而降低，而细菌群落多样性指数则相对稳定。聚类分析表明，连作年限是决定真菌群落结构的关键因素，而不是决定细菌群落结构的关键因素。变形菌门、酸细菌门、子囊菌门和担子菌门的相对丰度随单作年限的增加而降低。与健康土样相比，枯萎病病株土样中有益细菌的丰度随时间的延长而显著降低。这些观察结果表明，长期连作后土壤微生物群落结构发生变化，包括有益微生物数量减少和病原真菌的积累，可能是导致土壤虚弱和莲藕枯萎病的原因。
Fusarium oxyporum f. sp. nelumbicola is a very important plant pathogen that can cause lotus Fusarium wilt and lead to vast economic loss in Jiangxi Province, China. Sequencing data of 16S ribosomal RNA genes and internal transcribed spacer regions revealed that different bacterial and fungal communities were present in soil samples from healthy or Fusarium wilt-infected lotus fields with different years of continuous cropping, indicating that the long-term continuous cropping of lotus significantly altered the soil microbial communities. Fungal diversity indexes in the healthy soil samples increased with the years of monoculture, but decreased in the Fusarium wilt-infected soil samples, whereas bacterial community diversity in all the soil samples was relatively stable. A cluster analysis revealed that consecutive cropping time was a key factor for determining fungal community structure, but not for bacterial community structure. The relative abundances of Proteobacteria, Acidobacterial, Ascomycota, and Basidiomycota phyla decreased with the years of lotus monoculture. Compared with the healthy soil samples, the abundances of beneficial bacteria in the Fusarium wilt-infected soil samples significantly decreased with time. These observations indicate that soil weakness and lotus Fusarium wilt disease may be attributed to structural changes of soil microbial communities after long-term continuous cropping, and the changes include reduction of beneficial microorganisms and accumulation of fungal pathogens.