Multiplex single cell profiling of chromatin accessibility by combinatorial cellular indexing.
Multiplex single cell profiling of chromatin accessibility by combinatorial cellular indexing.
复制标题
DOI：
10.1126/science.aab1601
复制
发表时间：
2015-05-22
期刊：
影响因子：
--
通讯作者：
Shendure J
中科院分区：
文献类型：
--
作者：
Cusanovich DA;Daza R;Adey A;Pliner HA;Christiansen L;Gunderson KL;Steemers FJ;Trapnell C;Shendure J
Technical advances have enabled the collection of genome and transcriptome datasets with single-cell resolution. However, single-cell characterization of the epigenome has remained challenging. Furthermore, because cells must be physically separated prior to biochemical processing, conventional single-cell preparatory methods scale linearly. We applied combinatorial cellular indexing to measure chromatin accessibility in thousands of single cells per assay, circumventing the need for compartmentalization of individual cells. We report chromatin accessibility profiles from over 15,000 single cells and use these data to cluster cells on the basis of chromatin accessibility landscapes. We identify modules of coordinately regulated chromatin accessibility at the level of single cells both between and within cell types, with a scalable method that may accelerate progress towards a human cell atlas.
