Comparison of gingiva-derived and bone marrow mesenchymal stem cells for osteogenesis
Comparison of gingiva-derived and bone marrow mesenchymal stem cells for osteogenesis
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DOI：
10.1111/jcmm.14632
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发表时间：
2019-11-01
影响因子：
5.3
通讯作者：
Kuroda, Shinji
中科院分区：
文献类型：
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作者：
Sun, Quan;Nakata, Hidemi;Kuroda, Shinji
目前，骨髓被认为是间充质干细胞的主要来源；然而，也有一些缺点和局限性。与其他间充质干细胞（MSC）来源相比，龈源间充质干细胞（GMSCs）丰富且易于通过微创细胞分离技术获得。本研究从小鼠的牙龈和骨髓中分离培养MSCs。通过成骨、成脂和成软骨分化以及流式细胞术对其进行表征。与骨髓间充质干细胞（BMSCs）比较，采用CCK-8增殖试验评价其增殖能力。采用ALP染色、ALP测定、茜素红染色评价成骨分化程度。对ALP、OCN、OSX和Runx2进行RT-qPCR检测。结果表明，GMSCs比BMSCs具有更高的增殖能力。在成骨诱导后，与BMSCs相比，GMSCs ALP更阳性，形成更多的矿化结节。RT-qPCR结果显示，与BMSCs相比，GMSCs中ALP、OCN、OSX和Runx2的表达显著升高。此外，我们发现在成骨诱导过程中，GMSCs中cd90阳性细胞的数量高于BMSCs。这些结果表明，转基因干细胞可能是未来骨组织工程的一个有前景的来源。
Presently, bone marrow is considered as a prime source of mesenchymal stem cells; however, there are some drawbacks and limitations. Compared with other mesenchymal stem cell (MSC) sources, gingiva-derived mesenchymal stem cells (GMSCs) are abundant and easy to obtain through minimally invasive cell isolation techniques. In this study, MSCs derived from gingiva and bone marrow were isolated and cultured from mice. GMSCs were characterized by osteogenic, adipogenic and chondrogenic differentiation, and flow cytometry. Compared with bone marrow MSCs (BMSCs), the proliferation capacity was judged by CCK-8 proliferation assay. Osteogenic differentiation was assessed by ALP staining, ALP assay and Alizarin red staining. RT-qPCR was performed for ALP, OCN, OSX and Runx2. The results indicated that GMSCs showed higher proliferative capacity than BMSCs. GMSCs turned more positive for ALP and formed a more number of mineralized nodules than BMSCs after osteogenic induction. RT-qPCR revealed that the expression of ALP, OCN, OSX and Runx2 was significantly increased in the GMSCs compared with that in BMSCs. Moreover, it was found that the number of CD90-positive cells in GMSCs elevated more than that of BMSCs during osteogenic induction. Taking these results together, it was indicated that GMSCs might be a promising source in the future bone tissue engineering.