ULTRASTRUCTURAL LOCALIZATION OF CATALASE AND L-ALPHA-HYDROXY ACID OXIDASE IN MICROPEROXISOMES OF HYDRA
ULTRASTRUCTURAL LOCALIZATION OF CATALASE AND L-ALPHA-HYDROXY ACID OXIDASE IN MICROPEROXISOMES OF HYDRA
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DOI：
10.1177/24.8.956644
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发表时间：
1976-01-01
影响因子：
3.2
通讯作者：
HAND, AR
中科院分区：
文献类型：
--
作者：
HAND, AR
The ultrastructural localization of catalase and L-.alpha.-hydroxy acid oxidase (L.alpha.HAO) was studied in 2 spp. of hydra. [H. littoralis and H. pseudoligactis]. Diaminobenzidine reaction product of catalase activity was present in small round or elongated bodies resembling microperoxisomes in the epitheliomuscular, digestive and gland cells. They were closely related to the endoplasmic reticulum, and were often found in proximity to deposits of lipid and glycogen. Reaction product of L.alpha.HAO activity was also associated with the microperoxisomes. With rapidly oxidized substrates, such as L-lactic acid, reaction product diffused into the cytoplasm around the microperoxisomes. With slowly oxidized substrates, such as DL-.alpha.-hydroxyisovaleric acid, reaction product was restricted to the matrix of the microperoxisomes. No reaction product was present in the microperoxisomes in the absence of substrate or with D-lactic acid. The rate of substrate oxidation measured biochemically roughly paralleled the amount of cytochemical reaction product deposited with different substrates. Microperoxisome-like bodies reactive for L.alpha.HAO were also found in the epidermal cnidoblasts, but catalase could not be demonstrated in them. This study provides the 1st cytochemical evidence for the presence of an H2O2-producing oxidase in microperoxisomes.
