TRANSPLANTATION AND CELLULAR ENGINEERING: Adipose tissue mesenchymal stem cell expansion in animal serum‐free medium supplemented with autologous human platelet lysate
TRANSPLANTATION AND CELLULAR ENGINEERING: Adipose tissue mesenchymal stem cell expansion in animal serum‐free medium supplemented with autologous human platelet lysate
复制标题
移植和细胞工程：在补充有自体人血小板裂解物的无血清培养基中进行脂肪组织间充质干细胞扩增
作者：
Ivan Souza Blande;Vinícius Bassaneze;Carolina Lavini;K. Faé;J. Kalil;A. Miyakawa;I. Schettert;J. Krieger
背景：间充质干细胞(MSCs)已被考虑用于人类再生治疗，尤其是在物种间污染的情况下，安全的培养和扩增方案是必要的。人血小板裂解物(PL)已被建议作为MSC体外扩增过程中的动物血清替代品。在这项工作中，描述了一种简单而有效的方法来获得自体PL来代替动物血清在细胞培养中的应用。
BACKGROUND: Mesenchymal stem cells (MSCs) have been considered for human regenerative therapy applications, and safe culture and expansion protocols are needed especially in the context of interspecies contamination. Human platelet lysate (PL) has been proposed as animal serum substitute during in vitro MSC expansion. In this work, a simplified and efficient method to obtain autologous PL to replace animal serum in cell culture applications is described.