Genetic fusions of heat-labile toxoid (LT) and heat-stable toxin b (STb) of porcine enterotoxigenic Escherichia coli elicit protective anti-LT and anti-STb antibodies.
Genetic fusions of heat-labile toxoid (LT) and heat-stable toxin b (STb) of porcine enterotoxigenic Escherichia coli elicit protective anti-LT and anti-STb antibodies.
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猪产肠毒素大肠杆菌的不耐热类毒素 (LT) 和耐热毒素 b (STb) 的基因融合可引发保护性抗 LT 和抗 STb 抗体。
DOI：
10.1128/cvi.00095-10
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Francis,DavidH
中科院分区：
文献类型：
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作者：
Zhang,Weiping;Francis,DavidH
产肠毒素大肠杆菌（ETEC）相关性腹泻给世界范围内的养猪生产者造成了巨大的经济损失.引起猪腹泻尤其是断奶后腹泻的ETEC菌株大多产生热不稳定毒素（LT）和热稳定毒素B（ST B）。LT通常用于疫苗开发，但STb由于其免疫原性差而未被包括在内。作为猪腹泻的毒力因子，STb需要作为抗原用于开发广谱疫苗。在这项研究中，我们使用了来源于猪ETEC的LT类毒素（LTR 192 G [以下简称LT 192]），携带成熟的STb肽，用于LT 192-STb融合，以增强STb的免疫原性，用于潜在的疫苗应用。免疫家兔和猪均检测到抗LT和抗STb抗体。此外，当用STb阳性ETEC菌株攻毒时，免疫后备母猪所产的所有10头哺乳仔猪均保持健康，而未免疫后备母猪所产的9头仔猪中有7头发生中度腹泻。该研究表明，LT 192-STb融合增强了抗STb的免疫原性，并表明LT 192-STb融合抗原可用于未来针对猪ETEC腹泻的疫苗开发。
EnterotoxigenicEscherichia coli(ETEC)-associated diarrhea causes a substantial economic loss to swine producers worldwide. The majority of ETEC strains causing porcine diarrhea, especially postweaning diarrhea (PWD), produce heat-labile toxin (LT) and heat-stable toxin b (STb). LT is commonly used in vaccine development, but STb has not been included because of its poor immunogenicity. As a virulence factor in porcine diarrhea, STb needs to be included as an antigen for development of broad-spectrum vaccines. In this study, we used an LT toxoid (LTR192G[hereafter, LT192]) derived from porcine ETEC to carry a mature STb peptide for LT192-STb fusions to enhance STb immunogenicity for potential vaccine application. Anti-LT and anti-STb antibodies were detected in immunized rabbits and pigs. In addition, when challenged with an STb-positive ETEC strain, all 10 suckling piglets borne by immunized gilts remained healthy, whereas 7 out 9 piglets borne by unimmunized gilts developed moderate diarrhea. This study indicates that the LT192-STb fusion enhanced anti-STb immunogenicity and suggests the LT192-STb fusion antigen can be used in future vaccine development against porcine ETEC diarrhea.