5-sulfosalicylic Acid Dihydrate-Based Pretreatment for the Modification of Enzyme-Linked Immunoassay of Fluoroquinolones in Fishery Products
5-sulfosalicylic Acid Dihydrate-Based Pretreatment for the Modification of Enzyme-Linked Immunoassay of Fluoroquinolones in Fishery Products
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DOI：
10.1080/15321819.2015.1006330
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发表时间：
2015-01
影响因子：
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通讯作者：
Mengqi Cui;Hong Lin;Xiudan Wang;Limin Cao;Jianxin Sui
中科院分区：
文献类型：
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作者：
Mengqi Cui;Hong Lin;Xiudan Wang;Limin Cao;Jianxin Sui
开发了一种简单、快速的样品提取方法来测定 FQ。用 2% 的 5-磺基水杨酸二水合物提取渔业样品，直接分析提取物，无需任何进一步的纯化或清理程序。 FQ以2%的5-磺基水杨酸二水合物标准品测定，浓度范围为0.1~25.6 μg L−1，检测限（LOD）为0.1 μg L−1。来自渔业样品的基质干扰被 2% 的 5-磺基水杨酸二水合物消除，并且在蛋白质印迹中不与辣根过氧化物酶 (HRP) 标记的 IgG 相互作用。当用 2% 的 5-磺基水杨酸二水合物提取样品时，没有观察到明显的基质干扰。渔业肌肉中 FQ 的回收率在 10-50 μg kg−1 浓度范围内为 72.37-94.35%。该提取程序比传统 ELISA 提取程序更快、更简单，可作为一种省时且经济高效的渔业样品中 FQ 监测方法。
A simple, rapid sample extraction method for the determination of FQs was developed. Fishery samples were extracted with 2% of 5-sulfosalicylic acid dihydrate and the extracts were analyzed directly without any further purification or clean-up procedures. The FQs were determined with standards of 2% of 5-sulfosalicylic acid dihydrate in the concentration range of 0.1-25.6 μg L−1, and the limit of detection (LOD) was 0.1 μg L−1. The matrix interference originated from fishery samples was eliminated by 2% of 5-sulfosalicylic acid dihydrate and did not interact with horseradish peroxidase (HRP) labeled IgG in western blotting. No significant matrix interference was observed as samples extracted with 2% of 5-sulfosalicylic acid dihydrate. Recoveries of FQs in fishery muscle were between 72.37–94.35% in the concentrations range of 10–50 μg kg−1.This extraction procedure was much rapider and simpler to conventional ELISA extraction procedure and could be used as a time-saving and cost-effective method for FQs monitoring in fishery samples.