Role of S1P/S1PR3 axis in release of CCL20 from human bronchial epithelial cells.
Role of S1P/S1PR3 axis in release of CCL20 from human bronchial epithelial cells.
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DOI：
10.1371/journal.pone.0203211
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Nishimura Y
中科院分区：
文献类型：
--
作者：
Kawa Y;Nagano T;Yoshizaki A;Dokuni R;Katsurada M;Terashita T;Yasuda Y;Umezawa K;Yamamoto M;Kamiryo H;Kobayashi K;Nishimura Y
Sphingosine kinase phosphorylates sphingosine to generate sphingosine 1 phosphate (S1P) following stimulation of the five plasma membrane G-protein-coupled receptors. The objective of this study is to clarify the role of S1P and its receptors (S1PRs), especially S1PR3 in airway epithelial cells. The effects of S1P on asthma-related genes expression were examined with the human bronchial epithelial cells BEAS-2B and Calu-3 using a transcriptome analysis and siRNA of S1PRs. To clarify the role of CCL20 in the airway inflammation, BALB/c mice were immunized with ovalbumin (OVA) and subsequently challenged with an OVA-containing aerosol to induce asthma with or without intraperitoneal administration of anti-CCL20. Finally, the anti-inflammatory effect of VPC 23019, S1PR1/3 antagonist, in the OVA-induced asthma was examined. S1P induced the expression of some asthma-related genes, such as ADRB2, PTGER4, and CCL20, in the bronchial epithelial cells. The knock-down of SIPR3 suppressed the expression of S1P-inducing CCL20. Anti-CCL20 antibody significantly attenuated the eosinophil numbers in the bronchoalveolar lavage fluid (P<0.01). Upon OVA challenge, VPC23019 exhibited substantially attenuated eosinophilic inflammation. S1P/S1PR3 pathways have a role in release of proinflammatory cytokines from bronchial epithelial cells. Our results suggest that S1P/S1PR3 may be a possible candidate for the treatment of bronchial asthma.
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影响因子：
3.7
作者：
O'Sullivan MJ;Hirota N;Martin JG
通讯作者：
Martin JG
影响因子：
7.3
作者：
HENRY, PJ;RIGBY, PJ;GOLDIE, RG
通讯作者：
GOLDIE, RG
影响因子：
4.6
作者：
AMSTAD, P;REDDEL, RR;HARRIS, CC
通讯作者：
HARRIS, CC
影响因子：
3.3
作者：
Lin, Sheng-Zhang;Chen, Kang-Jie;Zheng, Shu-Sen
通讯作者：
Zheng, Shu-Sen
DOI：
10.1073/pnas.1217355110
发表时间：
2013-02-26
影响因子：
11.1
作者：
Dusaban, Stephanie S.;Purcell, Nicole H.;Brown, Joan Heller
通讯作者：
Brown, Joan Heller