Encapsulation of phage P22 DNA in vitro.
Encapsulation of phage P22 DNA in vitro.
复制标题
噬菌体 P22 DNA 的体外封装。
DOI：
10.1016/0042-6822(79)90508-7
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发表时间：
1979
期刊：
影响因子：
3.7
通讯作者：
D. Botstein
中科院分区：
文献类型：
--
作者：
A. Poteete;Virginia Jarvik;D. Botstein
噬菌体 P22 形态发生的主要步骤包括前头的形成、DNA 封装、头部稳定和尾部添加。 P22 DNA 封装和后续组装步骤可以在“DNA 供体提取物”中进行体外。该提取物由外壳蛋白基因中携带突变的诱导溶原制成。提取物中噬菌体的组装取决于 P22 前头和 ATP 的添加。它不依赖于蛋白质或DNA合成，也不依赖于体外基因重组。活性前头可以从被野生型噬菌体或携带前头形成后组装步骤所需基因突变的噬菌体感染的细胞中纯化。缺乏任何正常结构蛋白的前体是有缺陷的并且在该体外系统中不能被修复。
The major steps in phage P22 morphogenesis include formation of proheads, DNA encapsulation, head stabilization, and tail addition. P22 DNA encapsulation and later assembly steps can proceedin vitroin a “DNA donor extract.” This extract is made from an induced lysogen carrying mutations in the coat protein gene. Assembly of phage in the extract is dependent on addition of P22 proheads and ATP. It is not dependent on protein or DNA synthesis, or on genetic recombinationin vitro. Active proheads can be purified from cells infected with wild-type phage or phage carrying mutations in genes required for post-prohead formation assembly steps. Proheads lacking any of the normal structural proteins are defective and are not repaired in thisin vitrosystem.