Poly(ethylene glycol) grafting to poly(ether imide) membranes: influence on protein adsorption and thrombocyte adhesion.
Poly(ethylene glycol) grafting to poly(ether imide) membranes: influence on protein adsorption and thrombocyte adhesion.
复制标题
DOI：
10.1002/mabi.201300309
复制
发表时间：
2013-12
影响因子：
4.6
通讯作者：
A. Neffe;M. von Ruesten-Lange;S. Braune;K. Luetzow;T. Roch;K. Richau;F. Jung;A. Lendlein
中科院分区：
文献类型：
--
作者：
A. Neffe;M. von Ruesten-Lange;S. Braune;K. Luetzow;T. Roch;K. Richau;F. Jung;A. Lendlein
已知在光滑表面上接枝的线性聚乙二醇的链长和端基会影响蛋白质吸附和血小板粘附。本文探讨了已建立的结构函数关系是否可以转移到应用相关的粗糙表面。通过光谱学、表面亲水性的变化和表面屏蔽效应，证明了不同链长（Mn =1 kDa或10 kDa）和端基（甲氧基或羟基）的单氨基聚亚胺（PEI）膜的接枝功能化。表面功能化会导致BSA吸附减少，但不会导致纤维蛋白原吸附减少。与未经处理的PEI表面相比，血栓细胞粘附增加。最后，粗糙而不是光滑的聚合物或金表面应该作为相关模型进行研究。
The chain length and end groups of linear PEG grafted on smooth surfaces is known to influence protein adsorption and thrombocyte adhesion. Here, it is explored whether established structure function relationships can be transferred to application relevant, rough surfaces. Functionalization of poly(ether imide) (PEI) membranes by grafting with monoamino PEG of different chain lengths (Mn =1 kDa or 10 kDa) and end groups (methoxy or hydroxyl) is proven by spectroscopy, changes of surface hydrophilicity, and surface shielding effects. The surface functionalization does lead to reduction of adsorption of BSA, but not of fibrinogen. The thrombocyte adhesion is increased compared to untreated PEI surfaces. Conclusively, rough instead of smooth polymer or gold surfaces should be investigated as relevant models.