Automated analysis of embryonic gene expression with cellular resolution in C-elegans
Automated analysis of embryonic gene expression with cellular resolution in C-elegans
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DOI：
10.1038/nmeth.1228
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发表时间：
2008-08-01
期刊：
影响因子：
48
通讯作者：
Waterston, Robert H.
中科院分区：
文献类型：
--
作者：
Murray, John Isaac;Bao, Zhirong;Waterston, Robert H.
我们描述了一个系统，该系统允许在胚胎发生过程中连续以细胞分辨率自动分析秀丽隐杆线虫的报告基因表达。我们通过定义几种胚胎表达转录因子的报告基因的表达模式来证明其实用性。不变的细胞谱系允许自动对齐多个表达谱，从而可以直接比较不同基因报告基因的表达。我们还使用该系统来监测正常发育的扰动，包括细胞分裂时间和细胞命运的变化。该系统的系统应用可以揭示每个细胞在整个发育过程中的基因活性。
We describe a system that permits the automated analysis of reporter gene expression in Caenorhabditis elegans with cellular resolution continuously during embryogenesis. We demonstrate its utility by defining the expression patterns of reporters for several embryonically expressed transcription factors. The invariant cell lineage permits the automated alignment of multiple expression profiles, allowing direct comparison of the expression of different genes' reporters. We also used this system to monitor perturbations to normal development involving changes both in cell-division timing and in cell fate. Systematic application of this system could reveal the gene activity of each cell throughout development.