Primary structure of the immI immunity region of bacteriophage P22.
Primary structure of the immI immunity region of bacteriophage P22.
复制标题
噬菌体 P22 的 ImmI 免疫区的一级结构。
DOI：
10.1016/s0022-2836(83)80070-9
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发表时间：
1983
影响因子：
5.6
通讯作者：
Susskind,MM
中科院分区：
文献类型：
--
作者：
Sauer,RT;Krovatin,W;DeAnda,J;Youderian,P;Susskind,MM
测定了P22噬菌体immI免疫区的DNA序列。该区域包括编码P22抗阻遏蛋白的antgene和编码负调节抗阻遏蛋白合成的蛋白的mtandarcegene。我们纯化了抗阻遏蛋白，筛选了抗阻遏蛋白的胰蛋白酶肽，并测定了这些分子的氨基末端序列和氨基酸组成。这些数据，结合DNA序列，定位antgene和定义完整的氨基酸序列的抗阻遏物（300个残基）。通过对mnt-am343和arc-amH 1605突变进行测序，定位了mntandarc基因。预测Mnt和Arc蛋白是氨基酸序列同源的小的碱性多肽。Mnt蛋白与λ Cro蛋白也有很高的序列同源性，它们的转录起始于Pant启动子，而Mnt的转录起始于可能与Pant重叠的启动子。Mnt蛋白显然是通过与Pant转录起始点附近的操纵子位点结合而起作用的。
The DNA sequence of theimmI immunity region of bacteriophage P22 has been determined. This region includes theantgene, which encodes the P22 antirepressor protein, and themntandarcgenes, which encode proteins that negatively regulate antirepressor synthesis. We have purified antirepressor protein and selected tryptic peptides of antirepressor, and have determined the amino terminal sequences and amino acid composition of these molecules. These data, in combination with the DNA sequence, locate theantgene and define the complete amino acid sequence of antirepressor (300 residues). Themntandarcgenes have been located by sequencing themnt-am343 andarc-amH1605 mutations. The Mnt and Arc proteins are predicted to be small, basic polypeptides that are homologous in amino acid sequence. The Mnt protein also shows significant sequence homology with the λ Cro protein.Thearcandantgenes are transcribed rightward from the Pantpromoter, whilemntis transcribed leftward from a promoter that may overlap Pant. The Mnt protein apparently acts by binding to an operator site located immediately adjacent to the startpoint of Panttranscription.