Natural Variation in OsLG3 Increases Drought Tolerance in Rice by Inducing ROS Scavenging.
Natural Variation in OsLG3 Increases Drought Tolerance in Rice by Inducing ROS Scavenging.
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OsLG3 的自然变异通过诱导 ROS 清除来提高水稻的耐旱性。
DOI：
10.1104/pp.17.01492
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发表时间：
2018
期刊：
影响因子：
7.4
通讯作者：
Li Zichao
中科院分区：
文献类型：
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作者：
Xiong Haiyan;Yu Jianping;Miao Jinli;Li Jinjie;Zhang Hongliang;Wang Xin;Liu Pengli;Zhao Yan;Jiang Chonghui;Yin Zhigang;Li Yang;Guo Yan;Fu Binying;Wang Wensheng;Li Zhikang;Ali Jauhar;Li Zichao
提高水稻在干旱胁迫下的表现有可能显著影响水稻的生产力。在这里，我们报告，ERF家族转录因子OsLG3积极调节水稻耐旱性。在前期的工作中，我们发现OsLG 3对水稻粒长有积极的影响，而不影响稻米品质。在本研究中，我们发现在干旱胁迫条件下，OsLG 3在高地中的表达比在低地稻中更强。通过候选基因关联分析，我们发现OsLG 3启动子的自然变异与水稻种子萌发过程中对渗透胁迫的耐受性相关。OsLG 3的过表达显著提高了水稻对模拟干旱的耐受性，而OsLG 3的抑制则导致水稻对模拟干旱的敏感性增强。系统进化分析表明，OsLG 3的耐旱等位基因可能提高了栽培粳稻的耐旱性。含有0sLG3IRAT109的优良等位基因的渐渗系和互补转基因系表现出增强的耐旱性，表明0sLG3的天然变异有助于水稻的耐旱性。进一步的研究表明，OsLG 3通过诱导活性氧自由基清除在水稻耐旱性中发挥积极作用。综上所述，我们的研究结果表明OsLG 3的自然变异有助于水稻的耐旱性，OsLG 3的优良等位基因是培育耐旱水稻品种的一个有希望的遗传资源。
Improving the performance of rice (Oryza sativa) under drought stress has the potential to significantly affect rice productivity. Here, we report that the ERF family transcription factor OsLG3 positively regulates drought tolerance in rice. In our previous work, we found thatOsLG3has a positive effect on rice grain length without affecting grain quality. In this study, we found thatOsLG3was more strongly expressed in upland rice than in lowland rice under drought stress conditions. By performing candidate gene association analysis, we found that natural variation in the promoter ofOsLG3is associated with tolerance to osmotic stress in germinating rice seeds. Overexpression ofOsLG3significantly improved the tolerance of rice plants to simulated drought, whereas suppression ofOsLG3resulted in greater susceptibility. Phylogenetic analysis indicated that the tolerant allele ofOsLG3may improve drought tolerance in cultivatedjaponicarice. Introgression lines and complementation transgenic lines containing the elite allele ofOsLG3IRAT109showed increased drought tolerance, demonstrating that natural variation inOsLG3contributes to drought tolerance in rice. Further investigation suggested thatOsLG3plays a positive role in drought stress tolerance in rice by inducing reactive oxygen species scavenging. Collectively, our findings reveal that natural variation inOsLG3contributes to rice drought tolerance and that the elite allele ofOsLG3is a promising genetic resource for the development of drought-tolerant rice varieties.