Rehmannia glutinosa induces glial cell line-derived neurotrophic factor gene expression in astroglial cells via cPKC and ERK1/2 pathways independently.
Rehmannia glutinosa induces glial cell line-derived neurotrophic factor gene expression in astroglial cells via cPKC and ERK1/2 pathways independently.
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DOI：
10.1016/j.phrs.2006.01.014
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发表时间：
2006-07
影响因子：
9.3
通讯作者：
Hai Yu;K. Oh‐hashi;Tatsuhide Tanaka;A. Sai;M. Inoue;Y. Hirata;K. Kiuchi
中科院分区：
文献类型：
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作者：
Hai Yu;K. Oh‐hashi;Tatsuhide Tanaka;A. Sai;M. Inoue;Y. Hirata;K. Kiuchi
在治疗痴呆的四种中药中，地黄（RG）可诱导C6胶质母细胞瘤细胞和原代培养的星形胶质细胞表达胶质细胞源性神经营养因子（GDNF）基因。全特异性蛋白激酶C（PKC）抑制剂（Ro-31-8220）和MAPK/ERK激酶1（MEK 1）抑制剂（U 0126）的存在完全减弱了RG诱导的C6胶质母细胞瘤细胞中GDNF mRNA的上调。常规PKC抑制剂（Gö 6976）也显著降低GDNF基因诱导。另一方面，发现RG处理刺激细胞外信号调节激酶1和2（ERK 1/2）的磷酸化，其先于C6胶质母细胞瘤细胞中GDNF mRNA的诱导。然而，没有一种PKC抑制剂显著改变RG刺激的ERK 1/2磷酸化。因此，RG刺激的GDNF基因表达可通过cPKC和ERK 1/2途径独立上调C6胶质母细胞瘤细胞。
Among four herbs of traditional Chinese medicines (TCMs) used in the therapy of dementia, Rehmannia glutinosa (RG) was found to induce the gene expression of glial cell line-derived neurotrophic factor (GDNF) in C6 glioblastoma cells and primary cultured astrocytes. The RG-induced GDNF mRNA up-regulation in C6 glioblastoma cells was completely attenuated by the presence of a pan-specific protein kinase C (PKC) inhibitor (Ro-31-8220) and a MAPK/ERK kinase 1 (MEK1) inhibitor (U0126). A conventional PKC inhibitor (Gö6976) also significantly decreased GDNF gene induction. On the other hand, RG treatment was found to stimulate phosphorylation of extracellular signal-regulated kinase 1 and 2 (ERK1/2), which preceded GDNF mRNA induction in C6 glioblastoma cells. However, none of the PKC inhibitors significantly changed RG-stimulated ERK1/2 phosphorylation. Therefore, RG-stimulated GDNF gene expression could be independently up-regulated through cPKC and ERK 1/2 pathways in C6 glioblastoma cells.