ACE2 Receptor-Modified Algae-Based Microrobot for Removal of SARS-CoV-2 in Wastewater
ACE2 Receptor-Modified Algae-Based Microrobot for Removal of SARS-CoV-2 in Wastewater
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DOI：
10.1021/jacs.1c04933
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发表时间：
2021-07
影响因子：
15
通讯作者：
Fangyu Zhang;Zhengxing Li;Lu Yin;Qiangzhe Zhang;Nelly Askarinam;Rodolfo Mundaca-Uribe;Farshad Tehrani;Emil Karshalev;Weiwei Gao;Liangfang Zhang;Joseph Wang
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作者：
Fangyu Zhang;Zhengxing Li;Lu Yin;Qiangzhe Zhang;Nelly Askarinam;Rodolfo Mundaca-Uribe;Farshad Tehrani;Emil Karshalev;Weiwei Gao;Liangfang Zhang;Joseph Wang
冠状病毒SARS-CoV-2可以在废水中存活数天，具有水媒人类传播的潜在风险，因此在遏制病毒和减少其传播方面提出了挑战。在此，我们报告了一个积极的生物杂交microrobot系统，提供了高效的捕获和去除目标病毒从各种水生介质。基于藻类的微型机器人是通过使用点击化学功能化微藻与血管紧张素转换酶2（ACE 2）受体对SARS-CoV-2刺突蛋白。由此产生的ACE 2-藻类机器人在包括饮用水和河水在内的各种水生介质中显示出快速（>100 μm/s）和持久（>24 h）的自推进，无需外部燃料。ACE 2-藻类-机器人的这种移动提供了SARS-CoV-2刺突蛋白和SARS-CoV-2假病毒的有效“即时”去除。具体而言，主动生物混合微型机器人导致95%的病毒刺突蛋白去除率和89%的假病毒去除率，显著超过了诸如静态ACE 2-藻类和裸藻的对照组。这些结果表明，生物功能化藻类对去除病毒和其他环境威胁的废水相当有前途。
The coronavirus SARS-CoV-2 can survive in wastewater for several days with a potential risk of waterborne human transmission, hence posing challenges in containing the virus and reducing its spread. Herein, we report on an active biohybrid microrobot system that offers highly efficient capture and removal of target virus from various aquatic media. The algae-based microrobot is fabricated by using click chemistry to functionalize microalgae with angiotensin-converting enzyme 2 (ACE2) receptor against the SARS-CoV-2 spike protein. The resulting ACE2-algae-robot displays fast (>100 μm/s) and long-lasting (>24 h) self-propulsion in diverse aquatic media including drinking water and river water, obviating the need for external fuels. Such movement of the ACE2-algae-robot offers effective “on-the-fly” removal of SARS-CoV-2 spike proteins and SARS-CoV-2 pseudovirus. Specifically, the active biohybrid microrobot results in 95% removal of viral spike protein and 89% removal of pseudovirus, significantly exceeding the control groups such as static ACE2-algae and bare algae. These results suggest considerable promise of biologically functionalized algae toward the removal of viruses and other environmental threats from wastewater.