PHAGE AND HOST GENETIC-DETERMINANTS OF THE SPECIFIC ANTICODON LOOP CLEAVAGES IN BACTERIOPHAGE-T4-INFECTED ESCHERICHIA-COLI CTR5X
PHAGE AND HOST GENETIC-DETERMINANTS OF THE SPECIFIC ANTICODON LOOP CLEAVAGES IN BACTERIOPHAGE-T4-INFECTED ESCHERICHIA-COLI CTR5X
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DOI：
10.1016/0022-2836(86)90476-6
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发表时间：
1986-03-05
影响因子：
5.6
通讯作者：
SNYDER, L
中科院分区：
文献类型：
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作者：
KAUFMANN, G;DAVID, M;SNYDER, L
两种宿主tRNA种类的反密码子环切割发生在噬菌体T4感染的大肠杆菌CTr 5X中，CTr 5X是限制多核苷酸激酶（pnk）或RNA连接酶（rli）缺陷的噬菌体突变体的宿主菌株。裂解产物随突变体积累，但在野生型感染中通过多核苷酸激酶和RNA连接酶反应进一步加工。stp中的失活突变抑制E. coliCTr 5X，并且，如图所示，也消除了反密码子核酸酶，表明stp产物具有这种活性。我们还表明，存在其他抑制突变的pnk-（pseT 2）突变，似乎不影响反密码子核酸酶，不在STP。结果表明，E. coliCTr 5X，称为prr，决定pnk-或rli-突变体的限制。携带prr的转导子在感染后表现为反密码子核酸酶反应产物，这表明prr决定了这种活性的具体表现。然而，prr不编码易受反密码子核酸酶攻击的tRNA种类。
Anticodon loop cleavages of two host tRNA species occur in bacteriophage T4-infected Escherichia coli CTr5X, a host strain restricting phage mutants deficient in polynucleotide kinase (pnk) or RNA ligase (rli). The cleavage products accumulate with the mutants but are further processed in wt infection through polynucleotide kinase and RNA ligase reactions. Inactivating mutations in stp suppress pnk- or rli- mutations in E. coli CTr5X and, as shown here, also abolish the anticodon nuclease, implicating the stp product with this activity. We show also that there exist other suppressing mutations of a pnk- (pseT2) mutation that appear not to affect the anticodon nuclease and are not in stp. It has been shown that a single locus in E. coli CTr5X, termed prr, determines the restriction of pnk- or rli- mutants. A transductant carrying prr featured upon infection the anticodon nuclease reaction products, suggesting that prr determines the specific manifestation of this activity. However, prr does not encode the tRNA species that are vulnerable to the anticodon nuclease.