GENE-ENZYME RELATIONSHIPS IN HISTIDINE BIOSYNTHESIS IN BACILLUS SUBTILIS
GENE-ENZYME RELATIONSHIPS IN HISTIDINE BIOSYNTHESIS IN BACILLUS SUBTILIS
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DOI：
10.1128/jb.97.3.1444-1448.1969
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发表时间：
1969-01-01
影响因子：
3.2
通讯作者：
NESTER, EW
中科院分区：
文献类型：
--
作者：
CHAPMAN, LF;NESTER, EW
在组氨酸生物合成的10个步骤中，有9个步骤的突变体已经被分离并通过酶试验进行了鉴定。通过脱氧核糖核酸介导的转化，每个基因座都被定位到与aro簇和其他组氨酸基因座的关系上。编码该途径中的酶3、6和8的基因与该基因簇相连。一个主要的组氨酸连锁群由指定酶1、2、5、7和10的基因组成。编码该途径第9步的基因与任何其他已鉴定的组氨酸基因座无关。主要组氨酸簇松散地连接到CysBand，而不连接到任何与芳香氨基酸生物合成有关的基因座。
Mutants for 9 of the 10 steps in histidine biosynthesis have been isolated and identified by enzyme assay. Each locus has been mapped in relation to thearocluster and to other histidine loci by deoxyribonucleic acid-mediated transformation. The genes which code for enzymes 3, 6, and 8 of the pathway are linked to thearocluster. A major histidine linkage group is composed of the genes which specify enzymes 1, 2, 5, 7, and 10. The locus which codes for step 9 of the pathway is unlinked to any other identifiedhisloci. The major histidine cluster is loosely linked tocysBand is unlinked to any of the loci concerned with aromatic amino acid biosynthesis.