The Roles of Different CLE Domains in Arabidopsis CLE Polypeptide Activity and Functional Specificity
The Roles of Different CLE Domains in Arabidopsis CLE Polypeptide Activity and Functional Specificity
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DOI：
10.1093/mp/ssq021
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发表时间：
2010-07-01
期刊：
影响因子：
27.5
通讯作者：
Feldman, Lewis
中科院分区：
文献类型：
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作者：
Meng, Ling;Ruth, Kenneth C.;Feldman, Lewis
CLE（CLVATA 3/ESR相关）家族的植物多肽信号分子共享一个保守的14个氨基酸（aa）基序，称为CLE基序，最近的研究表明这足以在体外发挥CLE功能。在这项研究中，我们报告，拟南芥CLE蛋白可以在组织特异性的方式发挥作用，并确认一些CLE因子可以通过不同的受体。使用结构域交换，我们第一次表明，CLE基序可能决定了植物中蛋白质的功能组织特异性。然而，我们也提供了证据支持新的观点，CLE基序（14氨基酸）以外的序列有助于CLE功能和功能特异性在体内。此外，我们报告，从不同的CLE蛋白质的推定的信号肽的缺失完全灭活CLE功能在体内，而交换CLE信号肽与传统的信号肽从水稻富含甘氨酸的细胞壁蛋白也影响CLE功能。因此，我们提出CLE基序本身通过指示每个CLE肽与其最佳受体的直接识别和相互作用来确定其功能性组织特异性，而受体可以以组织特异性方式获得。另一方面，CLE基序外的序列可以通过影响CLE肽的加工来影响CLE功能，导致特定组织和/或细胞中CLE肽的可用性和/或丰度的变化。
The CLE (CLVATA3/ESR-related) family of plant polypeptide signaling molecules shares a conserved 14-amino-acid (aa) motif, designated the CLE motif, which recent studies suggest is sufficient for CLE function in vitro. In this study, we report that Arabidopsis CLE proteins can function in a tissue-specific manner and confirm some CLE factors can act through different receptors. Using domain swapping, we show for the first time that the CLE motif likely determines much of the functional tissue-specificity of the proteins in planta. However, we also provide evidence in support of the new view that sequences outside the CLE motif (14 aa) contribute to CLE function and functional specificity in vivo. Additionally, we report that deletion of the putative signal peptide from different CLE proteins completely inactivates CLE function in vivo, whereas exchanging the CLE signal peptides with a conventional signal peptide from a rice glycine-rich cell wall protein also influences CLE function. We thus propose that the CLE motif itself determines its functional tissue-specificity by dictating the direct recognition and interaction of each CLE peptide with its optimal receptor(s), whereas the receptor(s) may be available in a tissue-specific manner. On the other hand, the sequences outside the CLE motif may influence CLE function by affecting the processing of CLE peptides, resulting in a change in the availability and/or abundance of CLE peptides in specific tissues and/or cells.