Gln5 selectively monodansylated substance P as a sensitive tool for interaction studies with membranes.
Gln5 selectively monodansylated substance P as a sensitive tool for interaction studies with membranes.
复制标题
Gln5 选择性单丹磺酰化物质 P 作为与膜相互作用研究的敏感工具。
DOI：
10.1006/bbrc.1994.2190
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发表时间：
1994
影响因子：
3.1
通讯作者：
C. Abad
中科院分区：
文献类型：
--
作者：
C. Ferrándiz;E. Pérez;L. Braco;C. Abad
P物质（SP）是一种神经肽，在中枢和外周神经系统中具有多种重要的生物活性。利用SP中存在谷氨酰胺残基的优势，使用转谷氨酰胺酶（TGase）反应用荧光探针单丹西尸胺标记该肽，以研究SP与模型或天然膜之间的相互作用。尽管证实了肽的两个相邻谷氨酰胺可以在连续反应中充当TGase的底物，但优化了条件以选择性标记Gln5。发现这种荧光SP类似物采用与天然肽类似的环境依赖性构象，并证明其对豚鼠气管具有功能活性。荧光光谱法用于证明丹磺酰化SP在涉及与膜相互作用的研究中的潜在用途。
Substance P (SP) is a neuropeptide endowed with several important biological activities both in the central and peripheral nervous system. Taking advantage of the presence of glutamine residues in SP, the peptide was labelled with the fluorescent probe monodansylcadaverine using the transglutaminase (TGase)-reaction in order to study interactions between SP and model or natural membranes. Although it was verified that both adjacent glutamines of the peptide can act as substrate for TGase in a consecutive reaction, conditions were optimized to selectively label Gln5. This fluorescent SP analogue was found to adopt environment-dependent conformations similar to those of the natural peptide and proved to be functionally active on guinea pig trachea. Fluorescence spectroscopy was used to demonstrate the potential use of dansylated SP in studies involving interactions with membranes.