Quercetin protects HCT116 cells from Dichlorvos-induced oxidative stress and apoptosis
Quercetin protects HCT116 cells from Dichlorvos-induced oxidative stress and apoptosis
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DOI：
10.1007/s12192-015-0651-7
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发表时间：
2016-01-01
影响因子：
3.8
通讯作者：
Essefi, Salwa Abid
中科院分区：
文献类型：
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作者：
Ben Salem, Intidhar;Boussabbeh, Manel;Essefi, Salwa Abid
本研究旨在评估槲皮素（QUER），一种黄酮类化合物与众所周知的药理作用，对敌敌畏（DDVP）诱导的毒性在体外使用HCT 116细胞可能的保护作用。通过细胞活力、活性氧（ROS）生成、抗氧化酶活性、丙二醛（MDA）生成和DNA片段化来监测细胞毒性。通过测量线粒体跨膜电位（DeltaIm）和caspase活化来评估凋亡。结果表明，QUER预处理HCT116细胞2 h，可显著降低DDVP诱导的细胞死亡，抑制ROS的产生，调节过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性，降低MDA水平。线粒体膜电位、DNA断裂和半胱天冬酶活化的降低也被QUER减弱。这些结果表明，饮食QUER可以保护HCT 116细胞免受DDVP诱导的氧化应激和凋亡。
The present study was designed to assess the possible protective effects of Quercetin (QUER), a flavonoid with well-known pharmacological effects, against Dichlorvos (DDVP)-induced toxicity in vitro using HCT116 cells. The cytotoxicity was monitored by cell viability, reactive oxygen species (ROS) generation, anti-oxidant enzyme activities, malondialdehyde (MDA) production, and DNA fragmentation. The apoptosis was assessed through the measurement of the mitochondrial transmembrane potential (Delta Im) and caspase activation. The results indicated that pretreatment of HCT116 cells with QUER, 2 h prior to DDVP exposure, significantly decreased the DDVP-induced cell death, inhibited the ROS generation, modulated the activities of catalase (CAT) and superoxide dismutase (SOD), and reduced the MDA level. The reductions in mitochondrial membrane potential, DNA fragmentation, and caspase activation were also attenuated by QUER. These findings suggest that dietary QUER can protect HCT116 cells against DDVP-induced oxidative stress and apoptosis.