Dynamic expression reveals a two-step patterning of WUS and CLV3 during axillary shoot meristem formation in Arabidopsis
Dynamic expression reveals a two-step patterning of WUS and CLV3 during axillary shoot meristem formation in Arabidopsis
复制标题
动态表达揭示了拟南芥腋芽分生组织形成过程中 WUS 和 CLV3 的两步模式
DOI：
10.1016/j.jplph.2017.03.017
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发表时间：
2017-07-01
影响因子：
4.3
通讯作者：
Hu, Yuxin
中科院分区：
文献类型：
--
作者：
Xin, Wei;Wang, Zhicai;Hu, Yuxin
种子植物在叶腋产生腋窝分生组织（axillary meriystems， AM）的能力显著，然而，AM中干细胞生态位的动态建立在很大程度上是未知的。综合分析了拟南芥芽部干细胞生态位形成过程中决定芽部干细胞生态位的两个关键标记基因WUSCHEL （WUS）和CLAVATA3 （CLV3）的动态模式，发现在AM干细胞生态位建立过程中，WUS和CLV3发生了两步模式。我们对wus和clv3突变体的进一步研究表明，这种两步模式可能对AM祖细胞的维持和AM干细胞生态位的规范至关重要。这些数据为在AM形成过程中如何建立干细胞生态位提供了细胞学框架。
Seed plants have a remarkable capability to produce axillary meristems (AM) in the leaf axils, however, the dynamic establishment of a stem cell niche in AM is largely uncharacterized. We comprehensively examined the dynamic patterning of WUSCHEL (WUS) and CLAVATA3 (CLV3), the two key marker genes defining the shoot stem cell niches, during AM formation in Arabidopsis, and we found that a two-step patterning of WUS and CLV3 occurred during AM stem cell niche establishment. Our further work on the wus and clv3 mutants implicates that such two-step patterning is likely critical for the maintenance of AM progenitor cells and the specification of AM stem cell niche. These data provide a cytological frame for how a stem cell niche is established during AM formation.