Construction of an Integrated Pepper Map Using RFLP, SSR, CAPS, AFLP, WRKY, rRAMP, and BAC End Sequences
Construction of an Integrated Pepper Map Using RFLP, SSR, CAPS, AFLP, WRKY, rRAMP, and BAC End Sequences
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DOI：
10.1007/s10059-009-0002-6
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发表时间：
2009-01-01
影响因子：
3.8
通讯作者：
Kim, Byung-Dong
中科院分区：
文献类型：
--
作者：
Lee, Heung-Ryul;Bae, Ik-Hyun;Kim, Byung-Dong
图位克隆、标记辅助选择（MAS）和标记辅助育种（MAB）都需要具有高重复性标记的良好遗传图谱。对于图谱构建以及染色体分配，基于单拷贝PCR的标记的开发和图谱整合过程是必要的。在本研究中，132个标记（57个来自BAC末端序列的STS，13个来自RFLP的STS和62个SSR）是新开发的单拷贝型PCR标记。它们与我们实验室先前开发的1830个标记一起使用，用Joinmap 3.0程序构建了一个整合的图谱。该图谱包含12条染色体上的169个SSR标记、354个RFLP标记、23个BAC末端STS标记、6个RFLP STS标记、152个AFLP标记、51个WRKY标记和99个rRAMP标记。该图谱包含辣椒TF68'和辣椒C. chinense 'Habanero'）和两个种内（C. annuum 'CM334'和C. annuum 'Chilsungcho'）的辣椒种群。该图谱由805个种间标记（图距1858 cM）和745个种内标记（图距1892 cM）组成。所用的辣椒STS首先是从辣椒‘CM334’的BAC克隆的末端序列开发的。该图谱为辣椒遗传图谱的构建和辣椒染色体连锁群的定位提供了重要信息。
Map-based cloning to find genes of interest, marker-assisted selection (MAS), and marker-assisted breeding (MAB) all require good genetic maps with high reproducible markers. For map construction as well as chromosome assignment, development of single copy PCR-based markers and map integration process are necessary. In this study, the 132 markers (57 STS from BAC-end sequences, 13 STS from RFLP, and 62 SSR) were newly developed as single copy type PCR-based markers. They were used together with 1830 markers previously developed in our lab to construct an integrated map with the Joinmap 3.0 program. This integrated map contained 169 SSR, 354 RFLP, 23 STS from BAC-end sequences, 6 STS from RFLP, 152 AFLP, 51 WRKY, and 99 rRAMP markers on 12 chromosomes. The integrated map contained four genetic maps of two interspecific (Capsicum annuum TF68' and C. chinense 'Habanero') and two intraspecific (C. annuum 'CM334' and C. annuum 'Chilsungcho') populations of peppers. This constructed integrated map consisted of 805 markers (map distance of 1858 cM) in interspecific populations and 745 markers (map distance of 1892 cM) in intraspecific populations. The used pepper STS were first developed from end sequences of BAC clones from Capsicum annum 'CM334'. This integrated map will provide useful information for construction of future pepper genetic maps and for assignment of linkage groups to pepper chromosomes.