Near-Infrared Spectroscopic Evaluation of Single-Kernel Deoxynivalenol Accumulation and Fusarium Head Blight Resistance Components in Wheat
Near-Infrared Spectroscopic Evaluation of Single-Kernel Deoxynivalenol Accumulation and Fusarium Head Blight Resistance Components in Wheat
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DOI：
10.1094/cchem-03-15-0057-r
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发表时间：
2016-01-01
期刊：
影响因子：
2.4
通讯作者：
Dowell, Floyd E.
中科院分区：
文献类型：
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作者：
Peiris, Kamaranga H. S.;Bockus, William W.;Dowell, Floyd E.
以3个不同抗赤霉病水平的小麦品种为材料，研究了赤霉病（FHB）症状、单粒脱氧雪腐镰刀菌烯醇（DON）含量及DON含量在不同抗赤霉病品种籽粒中的分布。用近红外光谱法估计DON水平。每穗含DON小穗率在珠峰、卡尔92和Overley的点接种穗间分别为15.2%、49.7%和89.1%，差异显著。在点接种卡尔92和奥弗利穗中可见镰刀菌损坏的籽粒的百分比显着高于珠峰。然而，每穗含DON的小穗数和视觉镰刀菌受损的籽粒值喷雾接种穗之间的三个品种没有显着差异。在喷雾接种的穗中，籽粒中DON含量的变化范围为0 - 291.3ppm，而小穗位置中DON含量的变化是随机的。相反，点接种穗中接种小穗下小穗的DON水平在三个品种之间表现出显著差异。Overley籽粒中DON积累量最高。这种近红外光谱分析方法可以作为一种新的方法来评价小麦品种的赤霉病毒素积累的抗性。也可以评价其他抗性组分，例如对病原体感染的抗性和对病原体传播的抗性。
Fusarium head blight (FHB) symptoms, single-kernel deoxynivalenol (DON) levels, and distribution of DON levels among kernels in response to artificial FHB inoculation were investigated in three selected wheat cultivars that had different reported levels of FHB resistance. DON levels were estimated with near-infrared spectroscopy. The percentages of DON-containing spikelets per spike of 15.2, 49.7, and 89.1% were significantly different among point-inoculated spikes of Everest, Karl 92, and Overley, respectively. The percentage of visually Fusarium-damaged kernels in point-inoculated Karl 92 and Overley spikes was significantly higher than for Everest. However, the DON-containing spikelets per spike and visually Fusarium-damaged kernels values for spray-inoculated spikes were not significantly different among the three cultivars. In spray-inoculated spikes, DON levels in kernels ranged from 0 to 291.3 ppm, whereas the variation of DON levels in spikelet positions was random. In contrast, DON levels in spikelets below the inoculated spikelet in point-inoculated spikes showed marked differences among the three cultivars. Overley had the highest DON accumulation in kernels. This near-infrared spectroscopy method may be used as a novel way to evaluate wheat cultivars for FHB resistance to toxin accumulation. Other resistance components such as resistance to pathogen infection and resistance to pathogen spread may also be evaluated.