Low aneuploidy rate in early pregnancy loss abortuses from patients with polycystic ovary syndrome
Low aneuploidy rate in early pregnancy loss abortuses from patients with polycystic ovary syndrome
复制标题
DOI：
10.1016/j.rbmo.2016.04.006
复制
发表时间：
2016-07-01
影响因子：
4
通讯作者：
Zhou, Can-quan
中科院分区：
文献类型：
--
作者：
Wang, Qiong;Luo, Lu;Zhou, Can-quan
进行了一项前瞻性队列研究，以确定染色体非整倍体是否增加多囊卵巢综合征（PCOS）患者早期自然流产的风险。共随访了1461例IVF和胚胎移植后受孕的患者;招募了100例临床自然流产患者，其中32例患有PCOS，68例没有PCOS。2013年以前，遗传分析包括常规培养绒毛染色体核型分析和多重连接依赖探针扩增亚端粒分析结合荧光原位杂交; 2013年以来，采用基于阵列的比较基因组杂交技术结合染色体核型分析。PCOS组和非PCOS组的年龄、BMI、妊娠史、孕龄和总促性腺激素剂量无显著差异。在PCOS组中，28.1%的流产显示非整倍体，这是显着低于（P = 0.001）在非PCOS组（72.1%）。进一步的统计学分析控制了母亲的年龄表明，PCOS妇女的流产显着减少（P = 0.001）可能有染色体非整倍体。胚胎非整倍体在PCOS妇女早期自然流产中并不起重要作用。母体因素导致的子宫内膜病变更可能是PCOS患者早期自然流产风险增加的原因。(C)2016生殖保健有限公司由爱思唯尔有限公司出版。版权所有。
A prospective cohort study was conducted to determine whether chromosome aneuploidy increases the risk of early spontaneous abortions in patients with polycystic ovary syndrome (PCOS). A total of 1461 patients who conceived after IVF and embryo transfer were followed; 100 patients who had experienced clinical spontaneous abortion were recruited, 32 with PCOS and 68 without PCOS. Before 2013, genetic analysis comprised conventional cultured villus chromosome karyotyping and a multiplex ligation-dependent probe amplification subtelomere assay combined with fluorescence in-situ hybridization; since 2013, array-based comparative genomic hybridization technique combined with chromosome karyotyping has been used. Age, BMI, pregnancy history, gestational age and total gonadotrophin dosage did not differ significantly between the PCOS and non-PCOS groups. In the PCOS group, 28.1% of abortuses demonstrated aneuploidy, which was significantly lower (P = 0.001) than in the non-PCOS group (72.1%). Further statistical analyses controlling for maternal age demonstrated that abortuses of women with PCOS were significantly less (P = 0.001) likely to have chromosome aneuploidy. Embryonic aneuploidy does not play a vital role in early spontaneous abortion in women with PCOS. Maternal factors resulting in endometrial disorders are more likely to be responsible for the increased risk of early spontaneous abortion in patients with PCOS. (C) 2016 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.