Assessment of Th1/Th2 cytokines among patients with Middle East respiratory syndrome coronavirus infection
Assessment of Th1/Th2 cytokines among patients with Middle East respiratory syndrome coronavirus infection
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DOI：
10.1093/intimm/dxaa047
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发表时间：
2020-12-01
影响因子：
4.4
通讯作者：
Memish, Ziad A.
中科院分区：
文献类型：
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作者：
Alhetheel, Abdulkarim;Albarrag, Ahmed;Memish, Ziad A.
中东呼吸综合征冠状病毒（MERS-CoV）是起源于人畜共患的乙型冠状病毒属的一种，出现于阿拉伯半岛，与高发病率和死亡率相关。本研究采用定制的luminex试剂盒，对46例mers - cov感染患者（19例无症状和27例有症状）和52例正常健康对照进行血浆细胞因子水平评估，以评估T(h)1/T(h)2状态。MERS-CoV感染患者与正常健康对照的数据比较分析显示，尽管无症状MERS-CoV患者与对照组之间无差异，但平均血浆白细胞介素(IL)-10水平（44.69 +/- 40.04 pg ml（-1）与14.84 +/- 6.96 pg ml(-1)）；P < 0.0001), IL-4 (22.46 +/- 8.02 pg ml(-1) vs . 16.01 +/- 9.97 pg ml(-1)；P < 0.0001), IL-5 (10.78 + / - 2.86 pg毫升(1)和8.06 + / - 1.41 pg毫升(1);P < 0.0001)和IL-13 (14.51 +/- 3.97 pg ml(-1) vs . 11.53 +/- 4.16 pg ml(-1)；P < 0.003)，明显高于正常对照组。各研究组的平均血浆干扰素(IFN)- γ和IL-12水平无差异。有症状的mers - cov感染患者的细胞因子谱偏向T(h)2型免疫反应。
Middle East respiratory syndrome coronavirus (MERS-CoV) is a member of the beta-coronavirus genus of zoonotic origin that emerged in the Arabian Peninsula and is associated with significant morbidity and mortality. This study was conducted to assess the plasma levels of cytokines to evaluate the T(h)1/T(h)2 status among 46 MERS-CoV-infected patients (19 asymptomatic and 27 symptomatic) and 52 normal healthy controls using a customized luminex kit. Comparative analysis of data between MERS-CoV-infected patients and normal healthy controls revealed that although no difference was observed between asymptomatic MERS-CoV patients and controls, the mean plasma levels of interleukin (IL)-10 (44.69 +/- 40.04 pg ml(-1) versus 14.84 +/- 6.96 pg ml(-1); P < 0.0001), IL-4 (22.46 +/- 8.02 pg ml(-1) versus 16.01 +/- 9.97 pg ml(-1); P < 0.0001), IL-5 (10.78 +/- 2.86 pg ml(-1) versus 8.06 +/- 1.41 pg ml(-1); P < 0.0001) and IL-13 (14.51 +/- 3.97 pg ml(-1) versus 11.53 +/- 4.16 pg ml(-1); P < 0.003) in MERS-CoV symptomatic patients were significantly higher than the normal controls. The mean plasma levels of interferon (IFN)-gamma and IL-12 were no different among the study groups. The cytokine profile among symptomatic MERS-CoV-infected patients was skewed to a T(h)2 type immune response.