Prevalence of antibodies to human herpesvirus 8 (HHV-8) in Saudi Arabian patients with and without renal failure.
Prevalence of antibodies to human herpesvirus 8 (HHV-8) in Saudi Arabian patients with and without renal failure.
复制标题
患有或不患有肾衰竭的沙特阿拉伯患者中人类疱疹病毒 8 (HHV-8) 抗体的流行情况。
DOI：
10.1097/00007890-200104270-00019
复制
发表时间：
2001
期刊：
影响因子：
6.2
通讯作者：
Rigsby,MO
中科院分区：
文献类型：
--
作者：
Almuneef,M;Nimjee,S;Khoshnood,K;Miller,G;Rigsby,MO
背景：卡波西肉瘤（KS）是沙特阿拉伯肾移植受者最常见的癌症，其移植后KS的报告发病率比美国和西欧高10倍。在这个地理区域移植后KS发病率特别高的原因是未知的。方法：探讨移植后KS的高发病率可能是人类疱疹病毒8型（HHV-8）广泛感染的结果的可能性，我们测定了201例终末期肾病（ESRD）患者的HHV-8抗体流行率，对照组为358名年龄、性别和居住地区相似的无肾脏疾病的个体。用间接免疫荧光法检测HHV-8裂解周期抗原抗体，并用十四酰佛波酯诱导的BC-3细胞提取物和重组小病毒衣壳抗原（ORF 65）进行免疫印迹证实ESRD组201例中有10例（6.97%），对照组258例中有10例（3.88%），两组比较差异有统计学意义（P= 0.14）。HHV-8血清阳性个体平均比血清阴性受试者大10岁（55.3岁对46.9岁）。在HHV-8血清学阳性受试者中，71%为男性，29%为女性。结论：HHV-8感染的血清学证据在男性和ESRD患者中更常见。然而，在有和没有ESRD的患者中，最强的相关性是随着年龄的增长。
Background.Kaposi’s sarcoma (KS) is the most common cancer occurring in renal transplant recipients in Saudi Arabia, where the reported incidence of posttransplantation KS is 10 times higher than the incidence in the United States and Western Europe. The reason for the particularly high incidence of posttransplantation KS in this geographic area is unknown.Methods.To explore the possibility that the high incidence of posttransplantation KS might be the result of widespread infection with human herpesvirus 8 (HHV-8), we determined the prevalence of antibodies to HHV-8 in 201 patients with end-stage renal disease (ESRD) and a comparison group of 358 individuals without renal disease who were similar in age, sex, and area of residence. Antibodies to lytic cycle antigens of HHV-8 were determined by indirect immunofluorescence and confirmed by immunoblots using tetradecanoyl phorbol ester acetate-induced BC-3 cell extracts and recombinant small viral capsid antigen (ORF65).Results.Antibodies to HHV-8 were detected in serum samples from 14 (6.97%) of 201 ESRD patients and from 10 (3.88%) of 258 in the comparison group (P= 0.14). HHV-8 seropositive individuals were on average 10 years older than seronegative subjects (55.3 years vs. 46.9 years). Among HHV-8 seropositive subjects, 71% were male and 29% were female.Conclusions.Serologic evidence of HHV-8 infection was numerically more common in men and in patients with ESRD. However, among patients with and without ESRD, the strongest association was with increasing age.