Functional and morphological differentiation of nonpigmented ciliary body epithelial cells grown on collagen rafts.
Functional and morphological differentiation of nonpigmented ciliary body epithelial cells grown on collagen rafts.
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在胶原筏上生长的非色素睫状体上皮细胞的功能和形态分化。
DOI：
10.1007/s11626-997-0097-z
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Fain,GL
中科院分区：
文献类型：
--
作者：
Cilluffo,MC;Farahbakhsh,NA;Fain,GL
我们研究了细胞形状改变对培养的睫状体无色素上皮细胞分化形态和生理的影响。我们在从培养皿中释放的胶原凝胶上培养了纯的无色素细胞群，以创建胶原筏。在凝胶脱离后不久，当细胞收缩凝胶时，细胞的直径缩小，高度增加。同时，肌动蛋白细胞骨架重组到细胞皮层，如在体内发现的。这种分化的形态发展后，细胞内Ca 2+的大的变化可以引起乙酰胆碱和肾上腺素或乙酰胆碱和生长抑素受体的同时激活，在完整的组织中看到。孤立的非色素细胞层的外植体培养物保持其肌动蛋白分布，也表现出协同Ca2+的增加。扩散细胞，生长在刚性基板上，有一个混乱的细胞骨架，很少表现出协同作用。这些数据表明，睫状体中协同Ca2+反应的机制在胶原筏上生长的非色素细胞中是有功能的。此外，这一途径似乎是敏感的细胞的细胞结构的处置。
We have examined the effect of alteration in cell shape on promoting differentiated morphology and physiology in cultured nonpigmented epithelial cells from the ciliary body. We have grown pure populations of nonpigmented cells on collagen gels released from the culture dish to create collagen rafts. Shortly after the gels were detached, the cells shrank in diameter and increased in height while they contracted the gel. Concurrently, the actin cytoskeleton reorganized to the cell cortex as foundin vivo. After this differentiated morphology developed, large changes in intracellular Ca2+could be elicited by simultaneous activation of acetylcholine and epinephrine or acetylcholine and somatostatin receptors as seen in intact tissue. Explant cultures of isolated nonpigmented cell layers maintained their actin distribution and also showed synergistic Ca2+increases. Spread cells, grown on rigid substrates, had a disorganized cytoskeleton and rarely showed synergism. These data suggest that the mechanism underlying synergistic Ca2+responses in the ciliary body is functional in nonpigmented cells grown on collagen rafts. In addition, this pathway appears to be sensitive to the disposition of the cell’s cytoarchitecture.