Genome-wide indel/SSR scanning reveals significant loci associated with excellent agronomic traits of a cabbage (Brassica oleracea) elite parental line '01-20'.
Genome-wide indel/SSR scanning reveals significant loci associated with excellent agronomic traits of a cabbage (Brassica oleracea) elite parental line '01-20'.
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DOI：
10.1038/srep41696
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发表时间：
2017-02-06
影响因子：
4.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Lv H;Wang Q;Han F;Liu X;Fang Z;Yang L;Zhuang M;Liu Y;Li Z;Zhang Y
优良亲本对作物育种具有重要意义。为了在甘蓝优良自交系‘01-20’中发现与优良经济性状相关的独特基因组座位，我们比较了‘01-20’及其五个姊妹系的表型和全基因组插入-缺失/简单序列重复序列。‘01-20’具有一系列优良的农艺性状，包括早熟、株型和叶色的改善。在全基因组水平上发现了与‘01-20’相似的另一个优良品系‘01-20’和‘01-07-258’的8个独特的基因座。此外，来自‘01-20’杂交组合的两个优良的双单倍体品系也遗传了这些座位。根据数量性状座位关联结果，发现了5个与重要农艺性状相关的基因座，这可以解释为什么优良亲本‘01-20’的外叶更绿，株型更紧凑挺拔，穗圆，芯长较短，口感更好。此外，其中一些座位对与不同性状相关的数量性状座位具有聚集性，因此对这些区域中的重要基因进行了分析。本研究结果为甘蓝分子标记辅助多性状选择在全基因组水平上的应用奠定了基础，并为研究甘蓝显着基因组座位及其育种效果提供了理论依据。
Elite parental lines are of great significance to crop breeding. To discover unique genomic loci associated with excellent economic traits in the elite cabbage inbred-line ‘01–20’, we performed comparisons of phenotypes as well as whole-genome insertion-deletion/simple sequence repeat loci between ‘01–20’ and each of its five sister lines. ‘01–20’ has a range of excellent agronomic traits, including early-maturing, and improvements in plant type and leaf colour. Eight unique loci were discovered for ‘01–20’ and ‘01-07-258’, another elite line similar to ‘01–20’ at the whole-genome level. In addition, two excellent double-haploid lines derived from a cross of ‘01–20’ also inherited these loci. Based on the quantitative trait locus association results, five of these loci were found to be associated with important agronomic traits, which could explain why the elite parent ‘01–20’ possesses greener outer leaves, a more compact and upright plant-type, rounder head, shorter core length, and better taste. Additionally, some of these loci have clustering effects for quantitative trait loci associated with different traits; therefore, important genes in these regions were analysed. The obtained results should enable marker-assisted multi-trait selection at the whole-genome level in cabbage breeding and provide insights into significant genome loci and their breeding effects.