Mosquito feeding-induced enhancement of Cache Valley virus (Bunyaviridae) infection in mice
Mosquito feeding-induced enhancement of Cache Valley virus (Bunyaviridae) infection in mice
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DOI：
10.1093/jmedent/35.3.261
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发表时间：
1998-05-01
影响因子：
2.1
通讯作者：
Beaty, BJ
中科院分区：
文献类型：
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作者：
Edwards, JF;Higgs, S;Beaty, BJ
Cache Valley (CV) 病毒是一种节肢动物传播的布尼亚病毒，最近已成为导致北美反刍动物不育和先天畸形的重要兽医病原体。为了研究媒介喂养对 CV 感染的作用，对成年小鼠皮下注射 CV、CV 和载体胸部提取物（媒介唾液来源），或将 CV 注射到强烈的、未感染蚊子进食的部位。小鼠注射CV或CV和载体唾液后并未被感染，也不会产生针对CV的抗体。然而，在感染后 2 周，将 CV 注射到蚊子进食部位会导致病毒血症并产生抗 CV 抗体。这种 CV 感染的增强是由三列伊蚊 (Aedes triseriatus (Say), Ae) 喂养后造成的。埃及伊蚊 (L.) 或淡色库蚊 (L.)。当蚊子进食后延迟 4 小时注射时，会出现增强作用，但当在远离蚊子进食的地点进行病毒注射时，不会观察到增强作用。这些结果表明，蚊子-脊椎动物宿主相互作用可能会增强虫媒病毒感染，并且虫媒病毒在节肢动物载体中的复制可能不是导致受感染节肢动物介导的感染毒力增加的原因。怀孕小鼠增强的CV感染不会导致不孕或畸形幼崽，这表明小鼠不是研究CV引起的畸形的合适模型。
Cache Valley (CV) virus, an arthropod-borne bunyavirus, recently has emerged as a significant veterinary pathogen causing infertility and congenital malformations in North American ruminants. To investigate the role of vector feeding on CV infection, adult mice were injected subcutaneously with CV, CV and vector thorax extract (a source of vector saliva), or CV into the site of intense, noninfected-mosquito feeding. Mice did not become infected after injection of CV or CV and vector saliva, nor did they produce antibodies to CV. However, injection of CV into sites of mosquito feeding resulted in viremia and production of anti-CV antibody by 2 wk after infection. This enhancement of CV infection resulted after feeding by Aedes triseriatus (Say),Ae. aegypti (L.), or Culex pipiens (L.). Enhancement occurred when injection was delayed up to 4 h after mosquito feeding, but it was not observed when virus injection was performed at a site distant from mosquito feeding. These results indicate that arbovirus infection may be enhanced by mosquito-vertebrate host interactions and that replication of arboviruses in arthropod vectors may not be responsible for increased virulence of infections mediated by infected arthropods. Enhanced CV infection in pregnant mice did not result in infertility or malformed pups, indicating that the mouse is not a suitable model to study CV-induced malformations.