Fog signaling has diverse roles in epithelial morphogenesis in insects
Fog signaling has diverse roles in epithelial morphogenesis in insects
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DOI：
10.7554/elife.47346
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发表时间：
2019-10-01
期刊：
影响因子：
7.7
通讯作者：
Roth, Siegfried
中科院分区：
文献类型：
--
作者：
Benton, Matthew Alan;Frey, Nadine;Roth, Siegfried
果蝇 Fog 通路代表了控制上皮形态发生的最了解的信号级联之一。在原肠胚形成过程中，雾会诱导顶端细胞收缩，从而驱动中胚层和后肠原基的内陷。昆虫原基内化的细胞机制差异很大，最近的研究表明，雾信号传导是某些果蝇中发现的原肠胚形成的快速模式所特有的。相反，我们在甲虫Tribolium（其发育在昆虫中具有广泛代表性）中表明，雾具有多种形态发生功能。它调节中胚层内化并控制参与肠道和胚胎外发育的大量后内折叠。此外，雾信号传导影响胚外浆膜中的胚盘细胞化、原始生殖细胞定位以及立方形到鳞状细胞形状的转变。与其他两种远缘昆虫物种的比较分析表明，Fog 在细胞化过程中的作用广泛保守，因此可能代表了该途径的祖先功能。
The Drosophila Fog pathway represents one of the best-understood signaling cascades controlling epithelial morphogenesis. During gastrulation, Fog induces apical cell constrictions that drive the invagination of mesoderm and posterior gut primordia. The cellular mechanisms underlying primordia internalization vary greatly among insects and recent work has suggested that Fog signaling is specific to the fast mode of gastrulation found in some flies. On the contrary, here we show in the beetle Tribolium, whose development is broadly representative for insects, that Fog has multiple morphogenetic functions. It modulates mesoderm internalization and controls a massive posterior infolding involved in gut and extraembryonic development. In addition, Fog signaling affects blastoderm cellularization, primordial germ cell positioning, and cuboidal-to-squamous cell shape transitions in the extraembryonic serosa. Comparative analyses with two other distantly related insect species reveals that Fog's role during cellularization is widely conserved and therefore might represent the ancestral function of the pathway.