Establishment and characterization of neoplastic cells from a malignant fibrous histiocytoma. A possible stem cell line
Establishment and characterization of neoplastic cells from a malignant fibrous histiocytoma. A possible stem cell line
复制标题
恶性纤维组织细胞瘤肿瘤细胞的建立和表征。
作者：
K. Shirasuna;Masaru Sugiyama;Tadashi Miyazaki
使用半固体琼脂方法，从恶性纤维组织细胞瘤中分离出三个肿瘤克隆。所有克隆在形态上仅代表一种具有与具有多个丝状伪足的组织细胞相似的精细结构的细胞。携带 Fc 和 C3 表面受体的克隆表现出明显的免疫吞噬作用。它们的酸性磷酸酶、非特异性酯酶和 α-1-抗胰蛋白酶呈阳性。这些克隆能够显示出生产胶原基质的潜力。此外，将每个克隆接种到裸鼠中导致产生具有多形性模式的恶性纤维组织细胞瘤。这些肿瘤在形态上由各种类型的细胞组成，如未成熟细胞、组织细胞样细胞、成纤维细胞样细胞和多核巨细胞。组织细胞的这些形态学改变发生在体外的所有三个克隆中。这些发现表明，从恶性纤维组织细胞瘤建立的克隆细胞是肿瘤组织细胞，能够形成各种类型的细胞，可能是干细胞。
Using the semisolid agar method, neoplastic three clones were isolated from a malignant fibrous histiocytoma. All clones represented morphologically only one type of cells having fine structure similar to that of histiocyte with multiple filopodia. The clones carrying Fc‐ and C3‐surface receptors showed marked immunophagocytosis. They were positive for acid phosphatase, nonspecific esterase, and alpha‐1‐antitrypsin. These clones were able to display the potential for production of collagenous matrix. Moreover, inoculations of the each clone into nude mice resulted in productions of malignant fibrous histiocytoma with pleomorphic pattern. These tumors were composed morphologically of various types of cells such as immature, histiocyte‐like, fibroblast‐like, and multinucleated giant cells. These morphologic alterations of histiocytes occurred in all of the three clones in vitro. These findings suggest that the cloned cells established from the malignant fibrous histiocytoma are neoplastic histiocytes with capability to form various types of cells, a possible stem cell.