Fetal thymus organ culture.
Fetal thymus organ culture.
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DOI：
10.1101/pdb.prot4808
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发表时间：
2007-08-01
期刊：
影响因子：
--
通讯作者：
Jenkinson, Eric J
中科院分区：
文献类型：
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作者：
Anderson, Graham;Jenkinson, Eric J
从它们的祖细胞中产生具有功能的t细胞涉及一系列的发育事件，包括增殖、分化和存活。t细胞的发育是一个非细胞自主的事件，需要与胸腺基质细胞相互作用。胎儿胸腺器官培养提供了一个体外系统，在这个系统中，分离的胚胎胸腺叶可以在培养中维持，从而可以研究t细胞的发育以及胸腺基质细胞的功能。该系统仍然是唯一的体外系统，支持t细胞发育的完整程序，包括发展中的t细胞受体库的阳性和阴性选择。对基本的胎儿胸腺器官培养系统的改进，如挂滴培养和重组胸腺器官培养，分别为分析胸腺定植和胸腺基质细胞功能提供了有用的系统。
INTRODUCTIONThe generation of functionally competent T-cells from their progenitors involves a series of developmental events including proliferation, differentiation, and survival. T-cell development is a non-cell-autonomous event, and requires interactions with thymic stromal cells. Fetal thymus organ cultures provide an in vitro system in which isolated embryonic thymus lobes can be maintained in culture, allowing the study of T-cell development as well as thymic stromal cell function. This system remains the only in vitro system that supports a complete program of T-cell development, including positive and negative selection of the developing T-cell receptor repertoire. Modifications of the basic fetal thymus organ culture system, such as hanging drop cultures and reaggregate thymus organ cultures, provide useful systems to analyze thymus colonization and thymic stromal cell function, respectively.