Carnosic acid protects biomolecules from free radical-mediated oxidative damage in vitro
Carnosic acid protects biomolecules from free radical-mediated oxidative damage in vitro
复制标题
鼠尾草酸在体外保护生物分子免受自由基介导的氧化损伤
DOI：
10.1007/s10068-013-0226-2
复制
发表时间：
2013
影响因子：
2.9
通讯作者：
Xuebo Liu
中科院分区：
文献类型：
--
作者：
Qisen Xiang;Qian Liu;Li Xu;Y. Qiao;Yutang Wang;Xuebo Liu
鼠尾草酸（CA），一种天然存在的儿茶酚型多酚二萜，对自由基诱导的脂质，蛋白质和DNA的氧化损伤的保护作用进行了评估。用Cu ~（2+）/H_2O_2诱导人血清白蛋白和大鼠肝匀浆氧化。以Fe ~（2+）/VitC和2，2 ′-偶氮二（2，4-二甲基戊腈）引发亚油酸（LA）过氧化反应。用2，2 ′-偶氮二（2-脒基丙烷）二盐酸盐（AAPH）对DNA进行氧化损伤。结果表明，CA能显著抑制各种自由基对蛋白质、LA和DNA的氧化损伤。此外，在Fe ~（2+）/VitC诱导的-LA过氧化反应体系中，CA的加入也抑制了氧化亚油酸对RAW 264.7细胞的毒性作用。这些结果表明，有可能使用CA来预防或治疗氧化应激相关的障碍和疾病。
The protective effects of carnosic acid (CA), a naturally occurring catechol-type poly-phenolic diterpene, against free radical-induced oxidative damage to lipids, proteins, and DNA were evaluated in this study. The oxidation of human serum albumin and rat liver homogenate were induced by Cu2+/H2O2. Fe2+/VitC and 2,2′-azobis(2,4-dimethylvaleronitrile) were used to initiate linoleic acid (LA) peroxidation. Oxidative damage of DNA was carried out using 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH). The results showed that CA significantly inhibited the oxidative damages to proteins, LA and DNA caused by various types of free radicals. Moreover, in the Fe2+/VitC induced-LA peroxidation reaction system, the addition of CA also inhibited the cytotoxic effects of oxidized linoleic acid on RAW 264.7 cells. These results suggest that it might be possible to use CA to prevent or treat the oxidative stress-related disorders and diseases.