Glycosylated ovine prolactin.
Glycosylated ovine prolactin.
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糖基化绵羊催乳素。
DOI：
10.1073/pnas.81.2.385
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发表时间：
1984
影响因子：
11.1
通讯作者：
Sinha,YN
中科院分区：
文献类型：
--
作者：
Lewis,UJ;Singh,RN;Lewis,LJ;Seavey,BK;Sinha,YN
从绵羊脑垂体腺中分离到催乳素的一种修饰，其中31位的天冬酰胺携带一个碳水化合物单位。序列和氨基酸分析确定了碳水化合物的连接点。在酸性水解物中发现了氨基葡萄糖，这表明碳水化合物是通过N-乙酰氨基葡萄糖连接的。糖基化的催乳素与刀豆蛋白A和小扁豆凝集素结合，并用甲基α-D-甘露糖苷洗脱。在十二烷基硫酸钠的凝胶电泳过程中，糖基化激素以Mr 25000蛋白的形式迁移；催乳素的Mr为23000。改良的催乳素的效价为20国际单位/毫克，大约相当于参考催乳素制剂效价的60%，当用鸽子作物囊试验测量时。在放射免疫分析中，糖基化形式只有绵羊催乳素34%的免疫反应性。
A modification of prolactin, in which the asparagine at position 31 carries a carbohydrate unit, was isolated from ovine pituitary glands. Sequence and amino acid analyses identified the point of linkage of the carbohydrate. Glucosamine was found in acid hydrolysates, an indication that the carbohydrate is attached through N-acetylglucosamine. The glycosylated prolactin binds to concanavalin A and lentil lectin and is eluted with methyl alpha-D-mannopyranoside. During gel electrophoresis in sodium dodecyl sulfate, the glycosylated hormone migrates as a Mr 25,000 protein; prolactin has a Mr of 23,000. The modified prolactin had a potency of 20 international units/mg, approximately equal to 60% the potency of a reference prolactin preparation when measured by the pigeon crop sac assay. In a radioimmunoassay, the glycosylated form had only 34% the immunoreactivity of ovine prolactin.
催乳素和致癌作用：第四届 Tenovus 研讨会，卡迪夫，1972 年，编辑。
影响因子：
2.7
作者：
A. Segaloff
通讯作者：
A. Segaloff