AGENT IN AEDES-AEGYPTI CELL LINE (PELEG) WHICH CAUSES FUSION OF AEDES-ALBOPICTUS CELLS
AGENT IN AEDES-AEGYPTI CELL LINE (PELEG) WHICH CAUSES FUSION OF AEDES-ALBOPICTUS CELLS
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DOI：
10.1016/0042-6822(75)90113-0
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发表时间：
1975-01-01
期刊：
影响因子：
3.7
通讯作者：
THOMAS, VL
中科院分区：
文献类型：
--
作者：
STOLLAR, V;THOMAS, VL
埃及伊蚊（Peleg）细胞系的细胞培养物含有一种能引起白纹伊蚊细胞明显合胞体形成的物质。使用空斑测定来测量A中细胞融合剂（CFA）的生长。在BHK、KB和Vero细胞中不能复制或引起融合。CFA对乙醚和脱氧胆酸盐敏感，在蔗糖速度梯度中的沉降速度比辛德毕斯病毒慢。初步证据表明CFA可能与B组披膜病毒相似。
Cell cultures of theAedes aegypti(Peleg) cell line contain an agent that causes marked syncytium formation ofAedes albopictuscells. A plaque assay was used to measure the growth of the cell fusing agent (CFA) inA. albopictuscultures, and it was found to have a latent period of about 12 hr. It failed to replicate in or cause fusion of BHK, KB or Vero cells. The CFA was sensitive to ether and to deoxycholate and sedimented in a sucrose velocity gradient more slowly than did Sindbis virus. The preliminary evidence suggests that the CFA may be similar to the group B togaviruses.