Evidence that the anti-obesity effect of conjugated linoleic acid is independent of effects on stearoyi-CoA desaturasel expression and enzyme activity
Evidence that the anti-obesity effect of conjugated linoleic acid is independent of effects on stearoyi-CoA desaturasel expression and enzyme activity
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DOI：
10.1016/j.bbrc.2004.01.087
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发表时间：
2004-03-12
影响因子：
3.1
通讯作者：
Pariza, MW
中科院分区：
文献类型：
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作者：
Kang, KW;Miyazaki, M;Pariza, MW
共轭亚油酸（CLA）的反式-10，顺式-12异构体可减少动物的体脂增加，并抑制3 T3-L1脂肪细胞中的硬脂酰辅酶A去饱和酶（SCD）活性。为了测试CLA的体脂减少是否由SCD 1介导，野生型和SCD 1缺失小鼠被喂食补充有0.2%反式-10，顺式-12（t10 c12）CLA的饮食4周。补充t10 c12 CLA的饮食显著降低了野生型和SCD 1缺失小鼠的体脂量。同样，t10 c12 CLA饮食降低血液甘油三酯和游离脂肪酸水平，无论SCD 1基因型。喂食t10 c12 CLA的小鼠在两种基因型中都表现出脂肪酸合成酶和解偶联蛋白2的mRNA表达增加。总之，t10 c12共轭亚油酸对减少体脂肪增加，血液参数和mRNA表达在SCD 1基因敲除小鼠和野生型小鼠的影响是相似的，表明t10 c12共轭亚油酸的抗肥胖作用可能是独立的这种共轭亚油酸异构体对SCD 1基因表达和酶活性的影响。(C)2004年爱思唯尔公司All rights reserved.
The trans-10,cis-12 isomer of conjugated linoleic acid (CLA) reduces body fat gain in animals and inhibits stearoyl-CoA desaturase (SCD) activity in 3T3-L1 adipocytes. To test whether CLA's body fat reduction is mediated by SCD1, wild-type and SCD1-null mice were fed diet supplemented with 0.2% trans-10,cis-12 (t10c12) CLA for 4 weeks. The t10c12 CLA-supplemented diet significantly reduced body fat mass in both wild type and SCD1-null mice. Similarly, t10c12 CLA diet decreased blood triglyceride and free fatty acid levels regardless of SCD1 genotypes. Mice fed t10c12 CLA exhibited increased mRNA expression of fatty acid synthase and uncoupling protein 2 in both genotypes. Taken together, the effects of t10c12 CLA on reduction of body fat gain, blood parameters, and mRNA expression in both SCD1-null mice and wild-type mice were similar, indicating that the anti-obesity effect of t10c12 CLA may be independent of the effects of this CLA isomer on SCD1 gene expression and enzyme activity. (C) 2004 Elsevier Inc. All rights reserved.