Reverse correlation of rapid calcium signals in the zebrafish optic tectum in vivo
Reverse correlation of rapid calcium signals in the zebrafish optic tectum in vivo
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DOI：
10.1016/j.jneumeth.2006.04.021
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发表时间：
2006-10-30
影响因子：
3
通讯作者：
Engert, Florian
中科院分区：
文献类型：
--
作者：
Ramdya, Pavan;Reiter, Bettina;Engert, Florian
反向相关技术提供了一种计算神经元输入/输出关系的定量方法。到目前为止，这些方法仅限于电记录的响应，因为未经处理的光信号通常缺乏必要的时间特性。我们试图克服这一障碍，因为结合反向相关与钙成像将提供一个强大的替代目前的方法，测量神经元的响应特性的非侵入性体内。我们用钙指示剂标记了斑马鱼的视顶盖，并测量了对全场随机闪烁光刺激的反应。尽管钙信号表现出缓慢的衰减动力学，但我们可以使用计算建模来表明这些迹线的正微分提取高频信息。在实验中，我们发现，以这种方式处理的钙信号是同步的同时测量的突触反应，并可用于与反向相关，以确定在斑马鱼视顶盖的神经元的时间过滤器。这些发现表明，钙对生理刺激的反应可以被处理以获得快速的信号信息，从而确定体内线性滤波器的特性。(c)2006 Elsevier B. V.保留所有权利。
Reverse correlation techniques provide a quantitative means of computing neuronal input/output relationships. Until now these methods have been limited to electrically recorded responses since unprocessed optical signals generally lack necessary temporal characteristics. We sought to overcome this barrier since combining reverse correlation with calcium imaging would afford a powerful alternative to current methods of measuring response properties of neurons non-invasively in vivo. We labeled zebrafish optic tecta with a calcium indicator and measured responses to a whole-field random flicker light stimulus. Although calcium signals exhibited slow decay kinetics, we could use computational modeling to show that the positive differential of these traces extracts high frequency information. Experimentally, we found that calcium signals processed in this way were synchronous with simultaneously measured synaptic responses and could be used with reverse correlation to determine temporal filters of neurons in the zebrafish optic tectum. These findings demonstrate that calcium responses to physiological stimulation can be processed to obtain rapid signal information and consequently to determine linear filter properties in vivo. (c) 2006 Elsevier B.V. All rights reserved.