Determination of Monosaccharide Composition of Eucalyptus globulus Wood by FTIR Spectroscopy
Determination of Monosaccharide Composition of Eucalyptus globulus Wood by FTIR Spectroscopy
复制标题
傅里叶变换红外光谱法测定蓝桉木的单糖成分
DOI：
10.1515/hf.2001.044
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发表时间：
2001
影响因子：
3.5
通讯作者：
H. Pereira
中科院分区：
文献类型：
--
作者：
J. Rodrigues;J. Puls;O. Faix;H. Pereira
摘要利用蓝桉木材的自然变异性对傅里叶变换红外光谱法进行校准和验证，以估计蓝桉木材中的单糖含量。共使用了38个来自种源试验的9年生树木样品。基于无提取物的干木材，组成范围为葡萄糖43.2%至59.5%，木糖9.4%至17.8%，半乳糖0.5%至5.4%，甘露糖0%至2.8%，鼠李糖0.3%至0.8%。总体而言，多变量分析的结果更好，R2增加，SEC和SEP误差更低，尽管单变量方法也给出了良好的拟合，具有高决定系数（R2），基于去除离群值后的剩余数据。FTIR技术可用于大规模育种计划，以测量木材单糖组成，与湿实验室方法相比，一旦对物种进行了可靠的校准，其工作量和时间都要少得多。
Summary FTIR spectroscopy was used to estimate monosaccharide content in Eucalyptus globulus wood, using the natural variability of the species for calibration and validation of the method . A total of 38 samples from 9 year old trees from provenance trials was used. The composition ranged for glucose from 43.2% to 59.5%, for xylose from 9.4% to 17.8%, for galactose from 0.5% to 5.4%, for mannose from 0% to 2.8%, and for rhamnose from 0.3% to 0.8%, based on extractive-free dry wood. The multivariate analysis gave in general, better results with increased R2, lower SEC and SEP errors, although the univariate method also gave good fits with high coefficient of determination (R2) based on remaining data once outliers were removed. FTIR techniques may be used in large scale breeding programmes to measure wood monosaccharide composition with considerably less effort and in shorter time than wet-lab methods, once a reliable calibration has been made for the species.