Induction of nitric oxide synthase activity in rodent brain following middle cerebral artery occlusion.
Induction of nitric oxide synthase activity in rodent brain following middle cerebral artery occlusion.
复制标题
大脑中动脉闭塞后啮齿动物大脑中一氧化氮合酶活性的诱导。
DOI：
10.1016/0304-3940(95)11752-i
复制
发表时间：
1995
影响因子：
2.5
通讯作者：
Moskowitz,MA
中科院分区：
文献类型：
--
作者：
Yoshida,T;Waeber,C;Huang,Z;Moskowitz,MA
L-[3 H]精氨酸在无钙条件下转化为L-[3 H]瓜氨酸可用于选择性测定脂多糖给药后6 h大鼠脾匀浆中诱导型一氧化氮合酶（NOS）的活性。使用类似的测定条件下，诱导型NOS活性的变化，测定缺血脑组织内2小时和7天之间的永久性大脑中动脉（MCA）闭塞在Sprague-Dawley大鼠和SV-129小鼠。总（组成型和诱导型）NOS活性测定在0.5 mM氯化钙的存在下。而总NOS活性在大鼠大脑中动脉闭塞后6和12小时显着下降到基线的16%和6%，诱导型NOS活性在闭塞后2天内仍然检测不到，在3天达到最大，并在7天下降到最大iNOS活性的10%以下。在小鼠中，总NOS活性下降后MCA闭塞，但诱导型NOS活性是不可检测的闭塞后2小时至4天。诱导型NOS活性产生的持续NO在MCA闭塞后2 - 4小时内不会导致缺血性损伤，但在某些但不是所有物种中，可能会导致缺血后2-4天的梗死成熟。
The conversion of l-[3H]arginine to l-[3H]citrulline in the absence of calcium can be used to assay selectively the activity of inducible nitric oxide synthase (NOS) in rat spleen homogenates 6 h after lipopolysaccharide administration. Using similar assay conditions, changes in inducible NOS activity were measured within ischemic brain tissue between 2 h and 7 days following permanent middle cerebral artery (MCA) occlusion in Sprague-Dawley rats and SV-129 mice. Total (constitutive and inducible) NOS activity was measured in the presence of 0.5 mM CaCl2. Whereas total NOS activity in rat decreased dramatically to 16% and 6% of baseline 6 and 12 h after MCA occlusion, inducible NOS activity remained undetectable before 2 days after occlusion, became maximal at 3 days, and decreased to less than 10% of maximal iNOS activity at 7 days. In the mouse, total NOS activity decreased after MCA occlusion but inducible NOS activity was undetectable from 2 h to 4 days after occlusion. Sustained NO production by inducible NOS activity does not contribute to ischemic injury within 24 h after MCA occlusion, but may contribute to infarct maturation 2–4 days after ischemia in some but not all species.