Cyclin E and survival in patients with breast cancer.
Cyclin E and survival in patients with breast cancer.
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DOI：
10.1056/nejmoa021153
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发表时间：
2002-11-14
影响因子：
158.5
通讯作者：
Bacus, SS
中科院分区：
文献类型：
--
作者：
Keyomarsi, K;Tucker, SL;Bacus, SS
背景：细胞周期蛋白E是细胞周期的调节因子，影响乳腺癌细胞的行为。我们调查是否在肿瘤中的细胞周期蛋白E水平与乳腺癌患者的生存率之间的关系。方法：从395例乳腺癌患者的肿瘤组织中检测细胞周期蛋白E，细胞周期蛋白D1，细胞周期蛋白D3，HER-2/neu癌基因与使用Western印迹分析。全长，低分子量，和总细胞周期蛋白E进行了测量。还对256个肿瘤进行了细胞周期蛋白E的免疫组化评估。我们寻找这些分子标记物水平与疾病特异性和总生存率之间的相关性。结果：中位随访时间为6.4年。无论腋窝淋巴结转移阴性或阳性，高水平的低分子量细胞周期蛋白E亚型，如蛋白质印迹法检测，与疾病特异性生存率密切相关（P
Background: Cyclin E, a regulator of the cell cycle, affects the behavior of breast-cancer cells. We investigated whether levels of cyclin E in the tumor correlated with survival among patients with breast cancer.Methods: Tumor tissue from 395 patients with breast cancer was assayed for cyclin E, cyclin D1, cyclin D3, and the HER-2/neu oncogene with the use of Western blot analysis. Full-length, low-molecular-weight, and total cyclin E were measured. Immunohistochemical assessments of cyclin E were also made of 256 tumors. We sought correlations between levels of these molecular markers and disease-specific and overall survival.Results: The median follow-up was 6.4 years. A high level of the low-molecular-weight isoforms of cyclin E, as detected by Western blotting, correlated strongly with disease-specific survival whether axillary lymph nodes were negative or positive for metastases (P