STUDIES ON THE ANTIGENS OF PARAMECIUM-AURELIA WITH THE AID OF FLUORESCENT ANTIBODIES
STUDIES ON THE ANTIGENS OF PARAMECIUM-AURELIA WITH THE AID OF FLUORESCENT ANTIBODIES
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DOI：
10.1099/00221287-17-1-68
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发表时间：
1957-01-01
期刊：
影响因子：
--
通讯作者：
KACSER, H
中科院分区：
文献类型：
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作者：
BEALE, GH;KACSER, H
通过荧光显微镜检查通过暴露于预先与荧光素缀合的抗血清而固定的整个草履虫，这使得可以研究细胞的外部部分。荧光出现在生物体整个表面周围的薄层中，以及纤毛聚集尖端的小球中。细胞核和细胞质是不可见的，但一些食物泡是明亮的荧光。在从一种血清型转化为另一种血清型的过程中观察到的副伤寒杆菌表明，抗原的变化均匀地发生在生物体周围。似乎固定抗原是一种覆盖生物体整个表面的液体物质，并且它不断地渗出到生物体游泳的水中。
Whole paramecia, immobilized by exposure to antiserum previously conjugated with fluorescein, were examined by fluorescence microscopy, which permitted a study of the external parts of the cell. Fluorescence appeared in a thin layer around the entire surface of the organisms, and in globules at the clumped tips of the cilia. The nuclei and cytoplasm were invisible, but a few food vacuoles were brightly fluorescent. Observations of paramecia in the process of transforming from one serotype to another indicated that the change in antigen occurred uniformly around the organism. It seems likely that the immobilization antigen is a fluid substance covering the whole surface of the organism, and that it is constantly being exuded into the water in which the organisms are swimming.